在整体式微通道装置中的DNA测序。

DNA sequencing in a monolithic microchannel device.

作者信息

Backhouse C, Caamano M, Oaks F, Nordman E, Carrillo A, Johnson B, Bay S

机构信息

PE Biosystems, Foster City, CA 94404, USA.

出版信息

Electrophoresis. 2000 Jan;21(1):150-6. doi: 10.1002/(SICI)1522-2683(20000101)21:1<150::AID-ELPS150>3.0.CO;2-5.

DOI:10.1002/(SICI)1522-2683(20000101)21:1<150::AID-ELPS150>3.0.CO;2-5

PMID:10634481

Abstract

We present 50 cm long microchannels in a monolithic device for high resolution, long read-length DNA sequencing. These devices were fabricated and bonded in borofloat glass using unconventional photolithography techniques with 48-188 independent, straight microchannels. The microchannel DNA separation was tested with POP-6 polymer and a DNA sequencing ladder separated at room temperature and 200 V/cm. Single-base resolution greater than 600 bases was achieved and the sequence base called to 640 bases with 98% accuracy. Under the same experimental conditions, the performance of the microchip was identical to a fused-silica capillary with similar cross-sectional area.

摘要

我们展示了一种用于高分辨率、长读长DNA测序的单片设备中的50厘米长微通道。这些设备是使用非传统光刻技术在硼硅酸盐玻璃中制造并键合而成的，具有48 - 188个独立的直微通道。使用POP - 6聚合物对微通道DNA分离进行了测试，在室温及200 V/cm的条件下分离了DNA测序梯。实现了大于600个碱基的单碱基分辨率，并且对640个碱基进行序列碱基调用时准确率达到98%。在相同实验条件下，该微芯片的性能与具有相似横截面积的熔融石英毛细管相同。