Péoc'h M, Le Duc G, Trayaud A, Farion R, Le Bas J F, Pasquier B, Rémy C
INSERM U438, Grenoble, France.
Anticancer Res. 1999 Jul-Aug;19(4B):3025-30.
It has been suggested that the switch to an angiogenic phenotype can separate the development of a tumor into two stages: the prevascular phase and the vascular phase. The purpose of the present work is to demonstrate the existence of an angiogenic switch in a longitudinal study of a brain tumor model during tumor growth by means of microvessel density measurements.
The study was performed on 32 rats bearing C6 glioma. At different stages of tumor growth, the histological aspects were described and sections were immunostained for factor VIII-related antigen in order to highlight microvessel endothelial cells. Microvessels were counted at 400 magnification for different areas (central non necrotic area, peripheral area, contralateral grey and white matter area), using image analysis software.
Vessel density was significantly higher at the tumor-brain interface than in the center of the tumor or in the contralateral cortex. The vessel density remains stable in the tumor during the first 3 weeks after cell implantation, after which a clear increase of vessel density can be observed.
The present study demonstrates the presence of an angiogenic switch which is concomitant with the development of necrosis and pseudopalisading pattern.
有人提出，向血管生成表型的转变可将肿瘤的发展分为两个阶段：血管前期和血管期。本研究的目的是通过微血管密度测量，在脑肿瘤模型肿瘤生长的纵向研究中证明血管生成开关的存在。
对32只携带C6胶质瘤的大鼠进行研究。在肿瘤生长的不同阶段，描述组织学特征，并对切片进行因子VIII相关抗原免疫染色，以突出微血管内皮细胞。使用图像分析软件在400倍放大倍数下对不同区域（中央非坏死区、周边区、对侧灰质和白质区）的微血管进行计数。
肿瘤-脑界面处的血管密度显著高于肿瘤中心或对侧皮质。细胞植入后的前3周内，肿瘤内的血管密度保持稳定，此后可观察到血管密度明显增加。
本研究证明了血管生成开关的存在，其与坏死和假栅栏状模式的发展同时出现。
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