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Biochem J. 2000 Feb 15;346 Pt 1(Pt 1):127-31.
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[Beta-enolase in blood plasma during open heart surgery--comparing with CK-MB and CA-III in blood plasma].
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Mice over-expressing the myocardial creatine transporter develop progressive heart failure and show decreased glycolytic capacity.心肌肌酸转运蛋白过表达的小鼠会发展为进行性心力衰竭，并表现出糖酵解能力下降。
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1
Adenosinetriphosphate-creatine transphosphorylase. I. Isolation of the crystalline enzyme from rabbit muscle.三磷酸腺苷-肌酸转磷酸酶。I. 从兔肌肉中分离出结晶酶。
J Biol Chem. 1954 Jul;209(1):191-201.
2
Presence of enolase in the M-band of skeletal muscle and possible indirect interaction with the cytosolic muscle isoform of creatine kinase.烯醇化酶在骨骼肌M带中的存在以及与肌酸激酶胞质肌同工型可能的间接相互作用。
Biochem J. 1999 Feb 15;338 ( Pt 1)(Pt 1):115-21.
3
Myofibrillar interaction of cytosolic creatine kinase (CK) isoenzymes: allocation of N-terminal binding epitope in MM-CK and BB-CK.胞质肌酸激酶（CK）同工酶的肌原纤维相互作用：MM-CK和BB-CK中N端结合表位的定位
J Cell Sci. 1998 May;111 ( Pt 9):1207-16. doi: 10.1242/jcs.111.9.1207.
4
Transformation of slow- or fast-twitch rabbit muscles after cross-reinnervation or low frequency stimulation does not alter the in vitro properties of their satellite cells.交叉神经支配或低频刺激后，慢肌或快肌兔肌肉的转变不会改变其卫星细胞的体外特性。
J Muscle Res Cell Motil. 1998 Jan;19(1):25-32. doi: 10.1023/a:1005396125746.
5
Mobility of creatine phosphokinase and beta-enolase in cultured muscle cells.培养肌细胞中肌酸磷酸激酶和β-烯醇化酶的迁移率
Biophys J. 1997 Nov;73(5):2667-73. doi: 10.1016/S0006-3495(97)78295-X.
6
Diffusion of fluorescently labeled macromolecules in cultured muscle cells.荧光标记大分子在培养肌肉细胞中的扩散
Biophys J. 1996 May;70(5):2327-32. doi: 10.1016/S0006-3495(96)79798-9.
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Biochemical characterization of the mouse muscle-specific enolase: developmental changes in electrophoretic variants and selective binding to other proteins.小鼠肌肉特异性烯醇化酶的生化特性：电泳变体的发育变化及与其他蛋白质的选择性结合
Biochem J. 1997 May 1;323 ( Pt 3)(Pt 3):791-800. doi: 10.1042/bj3230791.
8
Enzyme-enzyme interactions and metabolite channelling: alternative mechanisms and their evolutionary significance.酶-酶相互作用与代谢物通道化：替代机制及其进化意义。
Biochem J. 1994 Mar 1;298 ( Pt 2)(Pt 2):313-20. doi: 10.1042/bj2980313.
9
Metabolic channeling versus free diffusion: transition-time analysis.
Trends Biochem Sci. 1994 May;19(5):193-7. doi: 10.1016/0968-0004(94)90019-1.
10
Fringe pattern photobleaching, a new method for the measurement of transport coefficients of biological macromolecules.条纹图案光漂白法，一种测量生物大分子传输系数的新方法。
EMBO J. 1982;1(10):1233-8. doi: 10.1002/j.1460-2075.1982.tb00018.x.

骨骼肌细胞胞质溶胶中β-烯醇化酶与肌酸激酶之间的相互作用。

Interactions between beta-enolase and creatine kinase in the cytosol of skeletal muscle cells.

作者信息

Foucault G, Vacher M, Cribier S, Arrio-Dupont M

机构信息

Gènes et Protéines Musculaires, EP CNRS 1088, Centre scientifique d'Orsay, bâtiment 430, F 91405 Orsay cedex, France.

出版信息

Biochem J. 2000 Feb 15;346 Pt 1(Pt 1):127-31.

PMID:10657248
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1220831/
Abstract

We studied interactions in vivo between the cytosolic muscle isoform of creatine kinase (M-CK) and the muscle isoform of 2-phospho-D-glycerate hydrolyase (beta-enolase) in muscle sarcoplasm by incubating glycerol-skinned fibres with FITC-labelled beta-enolase in the presence or absence of free CK. A small amount of bound beta-enolase was observed in the presence of large concentrations of CK. The mobility of enolase was measured in cultured satellite cells by modulated-fringe-pattern photobleaching. FITC-labelled beta-enolase was totally mobile in both the presence and the absence of CK but its diffusion coefficient was slightly lower in the presence of CK. This suggests a weak interaction in vivo between enolase and CK.

摘要

我们通过在有无游离肌酸激酶（CK）的情况下，将用异硫氰酸荧光素（FITC）标记的2-磷酸-D-甘油酸水解酶（β-烯醇化酶）与甘油处理的肌纤维一起孵育，研究了肌浆中肌酸激酶的胞质肌肉同工型（M-CK）与β-烯醇化酶的肌肉同工型之间的体内相互作用。在高浓度CK存在的情况下，观察到少量结合的β-烯醇化酶。通过调制条纹图案光漂白法在培养的卫星细胞中测量烯醇化酶的迁移率。FITC标记的β-烯醇化酶在有无CK的情况下均完全可移动，但其扩散系数在CK存在时略低。这表明烯醇化酶与CK在体内存在弱相互作用。