Wistar-ST和Sprague-Dawley品系大鼠远端结肠非肾上腺素能、非胆碱能舒张介质的差异。

Differences in mediator of nonadrenergic, noncholinergic relaxation of the distal colon between Wistar-ST and Sprague-Dawley strains of rats.

作者信息

Okishio Y, Niioka S, Takeuchi T, Nishio H, Hata F, Takatsuji K

机构信息

Department of Veterinary Pharmacology, College of Agriculture, Sakai, Japan.

出版信息

Eur J Pharmacol. 2000 Jan 24;388(1):97-105. doi: 10.1016/s0014-2999(99)00856-0.

DOI:10.1016/s0014-2999(99)00856-0

PMID:10657552

Abstract

Participation of nitric oxide and vasoactive intestinal peptide (VIP) in electrical field stimulation-induced nonadrenergic, noncholinergic (NANC) relaxation of longitudinal muscle and in balloon distension-induced descending NANC relaxation of circular muscle were studied in the distal colon of Wistar-ST and Sprague-Dawley rats. The extent of the nitric oxide-mediated component was approximately 50% in longitudinal and circular muscle of Sprague-Dawley rats, whereas this component was absent in both muscles of Wistar-ST rats. The extent of the VIP-mediated component was approximately 40% in longitudinal muscle of Wistar-ST rats and circular muscle of Sprague-Dawley rats, whereas this component was absent in circular muscle of Wistar-ST rats and longitudinal muscle of Sprague-Dawley rats. In circular muscle of Sprague-Dawley rats, in which participation of both nitric oxide and VIP in the relaxation was suggested, inhibition of descending relaxation by N(G)-nitro-L-arginine (L-NOARG) together with VIP-(10-28) was similar to that by either of the antagonists, and exogenous VIP-induced relaxation was not affected by L-NOARG, but exogenous nitric oxide-induced relaxation was partly inhibited by VIP-(10-28). These results suggest a linkage of the pathways mediated by nitric oxide and VIP. In the immunohistochemical studies, nitric oxide synthase or VIP immunoreactive neurons were seen in the ganglia, primary internodal strands of the myenteric plexus and in the circular muscle layer. However, the overall appearance of immunoreactive cell bodies in the myenteric plexus and the numbers of immunoreactive fibers in the circular muscle layer appeared to be similar in Wistar-ST and Sprague-Dawley rats. These results suggest that mediators of NANC relaxation in the distal colon are different in different strains of rats, i.e., Wistar-ST and Sprague-Dawley, although no such difference was seen in immunohistochemical studies.

摘要

在Wistar-ST和Sprague-Dawley大鼠的远端结肠中，研究了一氧化氮和血管活性肠肽（VIP）参与电场刺激诱导的纵肌非肾上腺素能、非胆碱能（NANC）舒张以及球囊扩张诱导的环肌下行NANC舒张的情况。在Sprague-Dawley大鼠的纵肌和环肌中，一氧化氮介导成分的比例约为50%，而在Wistar-ST大鼠的这两种肌肉中该成分均不存在。在Wistar-ST大鼠的纵肌和Sprague-Dawley大鼠的环肌中，VIP介导成分的比例约为40%，而在Wistar-ST大鼠的环肌和Sprague-Dawley大鼠的纵肌中该成分均不存在。在Sprague-Dawley大鼠的环肌中，提示一氧化氮和VIP均参与舒张过程，N(G)-硝基-L-精氨酸（L-NOARG）与VIP-(10-28)联合使用对下行舒张的抑制作用与单独使用任一拮抗剂时相似，外源性VIP诱导的舒张不受L-NOARG影响，但外源性一氧化氮诱导的舒张部分受VIP-(10-28)抑制。这些结果提示一氧化氮和VIP介导的途径存在联系。在免疫组织化学研究中，在神经节、肌间神经丛的初级节间束以及环肌层中可见一氧化氮合酶或VIP免疫反应性神经元。然而，Wistar-ST和Sprague-Dawley大鼠肌间神经丛中免疫反应性细胞体的总体外观以及环肌层中免疫反应性纤维的数量似乎相似。这些结果表明，尽管在免疫组织化学研究中未观察到差异，但不同品系的大鼠（即Wistar-ST和Sprague-Dawley）远端结肠中NANC舒张的介质不同。