一种在高转移性细胞中上调的GTPase超家族新成员。

A new member of the GTPase superfamily that is upregulated in highly metastatic cells.

作者信息

Nakaji T, Kataoka T R, Watabe K, Nishiyama K, Nojima H, Shimada Y, Sato F, Matsushima H, Endo Y, Kuroda Y, Kitamura Y, Ito A, Maeda S

机构信息

Second Department of Pathology, Kobe University Medical School, Japan.

出版信息

Cancer Lett. 1999 Dec 1;147(1-2):139-47. doi: 10.1016/s0304-3835(99)00285-2.

DOI:10.1016/s0304-3835(99)00285-2

PMID:10660099

Abstract

Two sublines of B16 melanoma cells, F10 and BL6, are metastatic after intravenous injection, but only BL6 cells are metastatic after subcutaneous injection. We found a new member of the GTPase superfamily, namely TIB929, which displayed an induction of expression in BL6 cells. It conserved three consensus sequences for GTP-binding site motifs and showed a significant homology to the yeast Gtr2 gene throughout the coding sequence. TIB929 was expressed ubiquitously in human tumor cells, with a marked expression in highly metastatic cells. TIB929 was mapped on mouse chromosome 4D, syntenic to human chromosome 1p. The results suggested an involvement of TIB929 in malignant progression.

摘要

B16黑色素瘤细胞的两个亚系F10和BL6，经静脉注射后具有转移性，但只有BL6细胞经皮下注射后具有转移性。我们发现了GTP酶超家族的一个新成员，即TIB929，它在BL6细胞中表现出表达诱导。它保留了GTP结合位点基序的三个共有序列，并且在整个编码序列上与酵母Gtr2基因具有显著同源性。TIB929在人类肿瘤细胞中普遍表达，在高转移性细胞中表达明显。TIB929定位于小鼠4D染色体，与人类1号染色体同线。结果表明TIB929参与了恶性进展。