White A C, Robinson P, Okhuysen P C, Lewis D E, Shahab I, Lahoti S, DuPont H L, Chappell C L
Infectious Diseases Section, Dept. of Medicine, Baylor College of Medicine, Houston, TX 77030.
J Infect Dis. 2000 Feb;181(2):701-9. doi: 10.1086/315261.
To investigate the role of interferon (IFN)-gamma in human cryptosporidiosis, jejunal biopsies from experimentally infected volunteers and chronically infected AIDS patients were examined for IFN-gamma expression by in situ hybridization. IFN-gamma expression was compared with oocyst excretion, baseline serum anti-Cryptosporidium antibody, and symptoms. IFN-gamma mRNA was detected in biopsies from 13 of 26 volunteers after experimental infection but not in biopsies taken before C. parvum exposure or in biopsies from patients with AIDS-associated cryptosporidiosis. After challenge, 9 of 10 volunteers with baseline C. parvum antibody produced IFN-gamma, compared with 4 of 16 volunteers without baseline antibody (P<.01). Furthermore, IFN-gamma mRNA was detected in 9 of 13 volunteers who did not excrete oocysts, compared with 4 of 13 with organisms (P<.05). Thus, expression of IFN-gamma in the jejunum was associated with prior sensitization and absence of oocyst shedding. IFN-gamma production may explain the resistance to infection noted in sensitized persons but may not be involved in control of human primary infection.
为研究γ干扰素（IFN-γ）在人类隐孢子虫病中的作用，通过原位杂交检测了实验性感染志愿者和慢性感染艾滋病患者的空肠活检组织中IFN-γ的表达情况。将IFN-γ表达与卵囊排泄、基线血清抗隐孢子虫抗体及症状进行了比较。在26名志愿者中的13名实验性感染后的活检组织中检测到了IFN-γ mRNA，但在暴露于微小隐孢子虫之前采集的活检组织或艾滋病相关隐孢子虫病患者的活检组织中未检测到。攻击后，10名有基线微小隐孢子虫抗体的志愿者中有9名产生了IFN-γ，而16名无基线抗体的志愿者中有4名产生了IFN-γ（P<0.01）。此外，在13名未排泄卵囊的志愿者中有9名检测到了IFN-γ mRNA，而在有卵囊排出的13名志愿者中有4名检测到（P<0.05）。因此，空肠中IFN-γ的表达与先前的致敏作用及无卵囊排出有关。IFN-γ的产生可能解释了致敏个体中所观察到的对感染的抵抗力，但可能不参与人类原发性感染的控制。
