磷脂酶C-β2和-β3以及磷脂酰肌醇-3激酶γ在趋化因子介导的信号转导中的作用。

Roles of PLC-beta2 and -beta3 and PI3Kgamma in chemoattractant-mediated signal transduction.

作者信息

Li Z, Jiang H, Xie W, Zhang Z, Smrcka A V, Wu D

机构信息

Department of Pharmacology, University of Rochester, Rochester, NY 14642, USA.

出版信息

Science. 2000 Feb 11;287(5455):1046-9. doi: 10.1126/science.287.5455.1046.

DOI:10.1126/science.287.5455.1046

PMID:10669417

Abstract

The roles of phosphoinositide 3-kinase (PI3K) and phospholipase C (PLC) in chemoattractant-elicited responses were studied in mice lacking these key enzymes. PI3Kgamma was required for chemoattractant-induced production of phosphatidylinositol 3,4,5-trisphosphate [PtdIns (3,4,5)P3] and has an important role in chemoattractant-induced superoxide production and chemotaxis in mouse neutrophils and in production of T cell-independent antigen-specific antibodies composed of the immunoglobulin lambda light chain (TI-IglambdaL). The study of the mice lacking PLC-beta2 and -beta3 revealed that the PLC pathways have an important role in chemoattractant-mediated production of superoxide and regulation of protein kinases, but not chemotaxis. The PLC pathways also appear to inhibit the chemotactic activity induced by certain chemoattractants and to suppress TI-IglambdaL production.

摘要

在缺乏这些关键酶的小鼠中，研究了磷酸肌醇3激酶（PI3K）和磷脂酶C（PLC）在趋化因子引发的反应中的作用。PI3Kγ是趋化因子诱导产生磷脂酰肌醇3,4,5-三磷酸[PtdIns(3,4,5)P3]所必需的，并且在趋化因子诱导的小鼠中性粒细胞超氧化物产生和趋化作用以及由免疫球蛋白λ轻链组成的非T细胞依赖性抗原特异性抗体（TI-IglambdaL）的产生中起重要作用。对缺乏PLC-β2和-β3的小鼠的研究表明，PLC途径在趋化因子介导的超氧化物产生和蛋白激酶调节中起重要作用，但在趋化作用中不起作用。PLC途径似乎还抑制某些趋化因子诱导的趋化活性并抑制TI-IglambdaL的产生。