睾丸旁腺瘤样瘤间皮起源的免疫组化证据。
Immunohistochemical evidence for mesothelial origin of paratesticular adenomatoid tumour.
作者信息
Delahunt B, Eble J N, King D, Bethwaite P B, Nacey J N, Thornton A
机构信息
Departments of Pathology, Wellington School of Medicine, University of Otago, New Zealand.
出版信息
Histopathology. 2000 Feb;36(2):109-15. doi: 10.1046/j.1365-2559.2000.00825.x.
AIMS
To investigate the histogenesis of paratesticular adenomatoid tumour by use of immunohistochemical markers for a variety of carcinomas and mesothelioma.
METHODS AND RESULTS
Immunohistochemical staining of sections from 12 cases of paratesticular adenomatoid tumour was undertaken using primary antibodies to antigens expressed by benign epithelial cells and carcinoma (cytokeratin AE1/AE3, cytokeratin 34ssE12, epithelial membrane antigen, MOC-31, Ber-EP4, CEA, B72.3, LEA.135, Leu M1), stromal and vascular markers (vimentin, CD34, factor VIII), and mesothelioma-associated antigens (thrombomodulin, HBME-1, OC 125) and p53 protein. There was absence of immunohistochemical expression of epithelial/carcinoma markers MOC-31, Ber-EP4, CEA, B72.3, LEA.135, Leu M1 and to factor VIII and CD34. All tumours expressed cytokeratin AE1/AE3, epithelial membrane antigen and vimentin, with weak expression of cytokeratin 34ssE12 in 25% of tumours. Each tumour showed expression of thrombomodulin, HBME-1 and OC 125 in a membranous distribution. p53 protein expression was not detected.
CONCLUSIONS
The immunohistochemical profile of paratesticular adenomatoid tumour is strongly supportive of a mesothelial cell origin.
目的
通过使用针对多种癌和间皮瘤的免疫组化标志物，研究睾丸旁腺瘤样瘤的组织发生。
方法与结果
对12例睾丸旁腺瘤样瘤的切片进行免疫组化染色，使用针对良性上皮细胞和癌所表达抗原的一抗（细胞角蛋白AE1/AE3、细胞角蛋白34βE12、上皮膜抗原、MOC-31、Ber-EP4、癌胚抗原、B72.3、LEA.135、Leu M1）、间质和血管标志物（波形蛋白、CD34、因子VIII）、间皮瘤相关抗原（血栓调节蛋白、HBME-1、OC 125）以及p53蛋白。上皮/癌标志物MOC-31、Ber-EP4、癌胚抗原、B72.3、LEA.135、Leu M1以及因子VIII和CD34均无免疫组化表达。所有肿瘤均表达细胞角蛋白AE1/AE3、上皮膜抗原和波形蛋白，25%的肿瘤细胞角蛋白34βE12呈弱表达。每个肿瘤均显示血栓调节蛋白、HBME-1和OC 125呈膜性分布表达。未检测到p53蛋白表达。
结论
睾丸旁腺瘤样瘤的免疫组化特征强烈支持其起源于间皮细胞。
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