Vescio R A, Wu C H, Zheng L, Sheen D, Ma H, Liu J, Stewart A K, Ballester O, Noga S J, Rugo H, Freytes C, Stadtmauer E, Sahebi F, Tarantolo S, Stiff P, Schiller G J, White M, Jacobs C, DiPersio J, Anderson K C, Berenson J R
West LA VAMC/University of California, Los Angeles, CA 90073, USA.
Bone Marrow Transplant. 2000 Jan;25(2):153-60. doi: 10.1038/sj.bmt.1702113.
Human herpesvirus 8 (HHV-8), also known as Kaposi's sarcoma-associated herpesvirus (KSHV), has recently been identified within the bone marrow dendritic cells of multiple myeloma (MM) patients. This virus contains homologues to human cytokines such as IL-6 that could potentially stimulate myeloma cell growth and contribute to disease pathogenesis. Since mobilization chemotherapy may increase circulating dendritic cell numbers, we searched for HHV-8 in peripheral blood mononuclear cells (PBMCs) before and after mobilization chemotherapy given to MM patients. Furthermore, we determined if autograft purging using the CEPRATE SC device would reduce the percentage of HHV-8 infected stem cell products. Only two of the 39 PBMC samples collected prior to mobilization chemotherapy contained PCR detectable virus, yet nine of 37 PBMCs collected on the first day of leukapheresis had detectable HHV-8 (P = 0.016). HHV-8 was more frequently identified in autograft products before vs after Ceprate SC selection (40% vs 15%, P = 0.016). Although the role HHV-8 plays in myeloma pathogenesis remains unclear, these results imply that mobilization chemotherapy increases the numbers of circulating HHV-8-infected dendritic cells within the peripheral blood. In addition, CD34 selection of autograft products in MM patients may reduce the reintroduction of virally infected cells following high-dose chemotherapy. Bone Marrow Transplantation (2000) 25, 153-160.
人类疱疹病毒8型（HHV - 8），也被称为卡波西肉瘤相关疱疹病毒（KSHV），最近在多发性骨髓瘤（MM）患者的骨髓树突状细胞中被发现。这种病毒含有与人类细胞因子如白细胞介素-6的同源物，可能刺激骨髓瘤细胞生长并促进疾病发病机制。由于动员化疗可能会增加循环树突状细胞数量，我们在给予MM患者动员化疗前后，对外周血单个核细胞（PBMC）中进行HHV - 8的检测。此外，我们确定使用CEPRATE SC装置进行自体移植净化是否会降低HHV - 8感染的干细胞产品的比例。在动员化疗前采集的39份PBMC样本中，只有两份样本通过PCR检测到病毒，但在白细胞分离术第一天采集的37份PBMC中，有9份检测到HHV - 8（P = 0.016）。在Ceprate SC选择之前与之后，自体移植产品中HHV - 8的检出率更高（40%对15%，P = 0.016）。虽然HHV - 8在骨髓瘤发病机制中的作用仍不清楚，但这些结果表明动员化疗增加了外周血中循环的HHV - 8感染的树突状细胞数量。此外，对MM患者的自体移植产品进行CD34选择可能会减少大剂量化疗后病毒感染细胞的重新引入。《骨髓移植》（2000年）25卷，第153 - 160页 。
