[Application of mouse limb bud culture to study the influence of zinc on teratogenesis induced by cadmium].

In this study, an in vitro method of mouse limb bud culture in self-made rotator with continuous supplementation of gas mixture was employed in studying the teratogenic potential of cadmium and the influence of zinc on the teratogenesis induced by cadmium. Image analysis on the area and the form of the bone analgen of the cultured limb was used to evaluate quantitatively their teratogenic potentials. Different amounts of cadmium were directly added to culture medium. As cadmium concentrations were increased from 0.1 to 1.0 microgram/ml, the degree of morphogenetic differentiation and the area of the bone anlagen of limbs culture were significantly decreased. The paws and long bones were affected seriously. Cadmium had a greater effect on chondrogenic tissue than on soft tissue. Then various levels of Zn, together with cadmium (1.0 microgram/ml medium), were added into the culture media. As Zn concentrations increased from 1.0 to 10.0 micrograms/ml, the degree of morphogenetic differentiation and the area of cartilaginous bone anlagen of limbs culture were improved or increased. The long bones were better ameliorated as compared with the paw.