Orlando V
DIBIT HSR Biomedical Scientific Park, Via Olgettina 58, 20132 Milano, Italy.
Trends Biochem Sci. 2000 Mar;25(3):99-104. doi: 10.1016/s0968-0004(99)01535-2.
Gene regulation is a complex process. Numerous factors appear to be required for the accurate temporal and spatial regulation of each gene. Often these factors are assembled into multiprotein complexes, contributing to specific gene regulation events. Understanding how all these factors are organized in the chromosome and how their function is regulated in vivo is a challenging task. One of the most useful techniques for studying this level of gene regulation is the in vivo fixation by formaldehyde crosslinking of proteins to proteins and proteins to DNA, followed by immunoprecipitation of the fixed material.
基因调控是一个复杂的过程。每个基因的精确时空调控似乎都需要众多因素。这些因素常常组装成多蛋白复合物，参与特定的基因调控事件。了解所有这些因素在染色体中是如何组织的，以及它们在体内的功能是如何被调控的，是一项具有挑战性的任务。研究这种基因调控水平最有用的技术之一是通过甲醛将蛋白质与蛋白质、蛋白质与DNA进行交联，从而在体内固定，随后对固定材料进行免疫沉淀。
