面神经横断后神经元型一氧化氮合酶蛋白抑制剂mRNA的变化

Changes in mRNA of protein inhibitor of neuronal nitric oxide synthase following facial nerve transection.

作者信息

Che Y H, Tamatani M, Yamashita T, Gomi F, Ogawa S, Tohyama M

机构信息

Department of Anatomy and Neuroscience, Osaka University Medical School, Japan.

出版信息

J Chem Neuroanat. 2000 Jan;17(4):199-206. doi: 10.1016/s0891-0618(99)00046-0.

DOI:10.1016/s0891-0618(99)00046-0

PMID:10697246

Abstract

Protein inhibitor of neuronal nitric oxide synthase (PIN) is reported as the protein inhibiting neuronal nitric oxide synthase (nNOS) activity by preventing dimerization of nNOS. It was also reported that PIN inhibits the activity of all nitric oxide synthase (NOS) isozymes. We examined the effects of facial nerve transection on PIN mRNA and NOS expression by in situ hybridization for PIN mRNA and nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) staining. PIN mRNA was initially expressed and transiently increased from 3 to 5 days and returned to the basal level at 7 days after axotomy in the motoneurons of the facial nucleus. NADPH-d-positive motoneurons were found from 7 days post-operation in the facial nucleus. These results suggest that PIN may interact with NOS from 7 days post-operation.

摘要

据报道，神经元型一氧化氮合酶蛋白抑制剂（PIN）可通过阻止神经元型一氧化氮合酶（nNOS）二聚化来抑制其活性。也有报道称，PIN可抑制所有一氧化氮合酶（NOS）同工酶的活性。我们通过原位杂交检测PIN mRNA以及烟酰胺腺嘌呤二核苷酸磷酸黄递酶（NADPH-d）染色，研究了面神经横断对面神经核运动神经元中PIN mRNA和NOS表达的影响。在面神经核运动神经元中，轴突切断术后3至5天，PIN mRNA开始表达并短暂增加，术后7天恢复至基础水平。术后7天在面神经核中发现了NADPH-d阳性运动神经元。这些结果表明，术后7天PIN可能与NOS相互作用。