Tsunoda T, Tanimura H, Hotta T, Tani M, Iwahashi M, Tanaka H, Matsuda K, Yamaue H
Second Department of Surgery, Wakayama Medical School, Japan.
Anticancer Res. 1999 Nov-Dec;19(6B):5451-5.
CPT-11 is a comptothecin analogue which has shown a broad spectrum of strong antitumor effect against various cancers, including gastroenterological malignancies. In the present study, the antitumor effect of CPT-11 was determined by MTT assay for freshly isolated human gastric and colorectal cancer cells, especially highly purified tumor cells. Twenty-three patients with gastric cancer, and 32 patients with colorectal cancer were enrolled in this study. Three gastric and 3 colonic cancer cell lines were used to study the antitumor effect of CPT-11, and freshly isolated cancer cells from 3 patients with gastric cancer were investigated. The in vitro antitumor effect was tested by MTT assay, and showed % inhibition rate. CPT-11 and SN-38 showed the antitumor effect as a dose dependent matter for human gastric and colorectal tumor cells in vitro. From the results of chemosensitivity for freshly isolated gastric and colorectal tumor cells, antitumor effect of SN-38 was as strong as other conventional anticancer agents. It was demonstrated that the MTT assay was appropriate for the analysis of the antitumor effects of CPT-11 and SN-38, and that CPT-11 may be a worthwhile choice as an anticancer agent against gastric and colorectal cancer.
喜树碱-11（CPT-11）是一种喜树碱类似物，已显示出对包括胃肠道恶性肿瘤在内的各种癌症具有广泛的强效抗肿瘤作用。在本研究中，通过MTT法对新鲜分离的人胃癌和结肠癌细胞，尤其是高度纯化的肿瘤细胞，测定CPT-11的抗肿瘤作用。本研究纳入了23例胃癌患者和32例结肠癌患者。使用3种胃癌和3种结肠癌细胞系研究CPT-11的抗肿瘤作用，并对3例胃癌患者新鲜分离的癌细胞进行了研究。通过MTT法测试体外抗肿瘤作用，并显示出抑制率百分比。CPT-11和SN-38在体外对人胃癌和结肠肿瘤细胞显示出剂量依赖性的抗肿瘤作用。从新鲜分离的胃癌和结肠肿瘤细胞的化疗敏感性结果来看，SN-38的抗肿瘤作用与其他传统抗癌药物一样强。结果表明，MTT法适用于分析CPT-11和SN-38的抗肿瘤作用，并且CPT-11可能是作为抗胃癌和结肠癌的抗癌药物的一个值得选择的药物。
