Karimzadeh K, Morrison J, Zadeh H H
Department of Periodontology, University of Southern California-School of Dentistry, Los Angeles 90089, USA.
J Periodontal Res. 1999 Nov;34(8):445-56. doi: 10.1111/j.1600-0765.1999.tb02280.x.
The present study investigated the expression of different variable regions of T cell receptor beta-chain (V beta) among functional subsets of T cells, i.e. CD45RO+ (activated/memory), CD4+ and CD8+ in gingiva and peripheral blood of patients with periodontitis. Gingival tissue specimens (n = 25) and peripheral blood were procured from 18 patients with periodontitis during periodontal surgery or extraction. Single-cell suspensions of gingival tissues were made by enzymatic digestion. These cells were immunofluorescently labeled with a panel of monoclonal antibodies specific for 18 TCR V beta regions, in concert with markers for various T cell subsets. The cells were then analyzed with 3-color multivariate flow cytometry. Results demonstrated that a significantly higher proportion of T cells in gingiva expressed V beta 5.2 (0.0005), V beta 6 (0.0007) and V beta 9 (0.003) regions compared to those in peripheral blood. Comparison of CD45RO+ (activated/memory) and CD45RO- (naïve) subsets of gingival T cells revealed differences in the expression of TCR V beta regions. V beta 5.2 expression was significantly higher among CD45RO+ gingival T cells (p = 0.004), whereas V beta 14 expression was elevated among the CD45RO- subset relative to peripheral blood (p = 0.008). Analysis of TCR V beta region expression among CD4+ and CD8+ subsets did not reveal any statistically significant differences between gingiva and peripheral blood, although some V beta regions approached significance. Collectively, these results demonstrate that the T cell repertoire in the gingival compartment differs significantly from that in the peripheral blood. Furthermore, since the skewing of TCR V beta was observed among naïve, as well as activated/memory T cells, it is likely that both developmental and environmental factors are influential in shaping the gingival TCR repertoire in patients with periodontitis. Elucidation of the cause of the skewed expression of T cell receptors in gingiva can provide insights into the specificity of T cells in periodontitis.
本研究调查了牙周炎患者牙龈和外周血中T细胞功能亚群（即CD45RO+（活化/记忆）、CD4+和CD8+）中T细胞受体β链（Vβ）不同可变区的表达情况。在牙周手术或拔牙过程中，从18例牙周炎患者获取牙龈组织标本（n = 25）和外周血。通过酶消化制备牙龈组织的单细胞悬液。这些细胞用一组针对18个TCR Vβ区域的单克隆抗体进行免疫荧光标记，并结合各种T细胞亚群的标志物。然后用三色多参数流式细胞术对细胞进行分析。结果表明，与外周血相比，牙龈中表达Vβ5.2（0.0005）、Vβ6（0.0007）和Vβ9（0.003）区域的T细胞比例显著更高。牙龈T细胞的CD45RO+（活化/记忆）和CD45RO-（初始）亚群的比较揭示了TCR Vβ区域表达的差异。CD45RO+牙龈T细胞中Vβ5.2的表达显著更高（p = 0.004），而相对于外周血，CD45RO-亚群中Vβ14的表达升高（p = 0.008）。CD4+和CD8+亚群中TCR Vβ区域表达的分析未发现牙龈和外周血之间有任何统计学上的显著差异，尽管一些Vβ区域接近显著水平。总体而言，这些结果表明牙龈区室中的T细胞库与外周血中的T细胞库有显著差异。此外，由于在初始以及活化/记忆T细胞中均观察到TCR Vβ的偏移，发育和环境因素可能都对塑造牙周炎患者的牙龈TCR库有影响。阐明牙龈中T细胞受体表达偏移的原因可以为牙周炎中T细胞的特异性提供见解。
Zotero 插件