Chandre F, Manguin S, Brengues C, Dossou Yovo J, Darriet F, Diabate A, Carnevale P, Guillet P
LIN/IRD, Montpellier, France.
Parassitologia. 1999 Sep;41(1-3):319-22.
In the field, the kdr mutation, involved in pyrethroid resistance, has been found widely distributed in the Savanna form of Anopheles gambiae s.s., but never in wild populations of the Mopti form or An. arabiensis, even in areas where both occur in sympatry with resistant Savanna populations. Under laboratory conditions, Mopti and Savanna forms were fully able to interbreed and the kdr mutation was transmissible from one form to the other. Both forms appeared to be exposed to pyrethroid selection pressure in the field. The absence of the kdr mutation in the Mopti form and the total lack of Mopti-Savanna heterozygotes in field populations provides further evidence of a pre-copulatory barrier to gene flow between these two forms. Molecular markers, including kdr, are powerful tools for studying population genetics and circulation of resistance genes, and should be used through an integrated approach for a better understanding of the speciation process.
在野外，参与拟除虫菊酯抗性的kdr突变已在冈比亚按蚊指名亚种的稀树草原形态中广泛分布，但在莫普提形态或阿拉伯按蚊的野生种群中从未发现，即使在这两种形态与具有抗性的稀树草原种群同域分布的地区也是如此。在实验室条件下，莫普提形态和稀树草原形态能够完全杂交，并且kdr突变能够从一种形态传递到另一种形态。这两种形态在野外似乎都受到拟除虫菊酯选择压力的影响。莫普提形态中不存在kdr突变以及野外种群中完全缺乏莫普提-稀树草原杂合子，这进一步证明了这两种形态之间存在交配前的基因流动障碍。包括kdr在内的分子标记是研究种群遗传学和抗性基因传播的有力工具，应该通过综合方法使用，以便更好地理解物种形成过程。
