Chen C, Yan H, Zhai W, Zhu L, Sun J
Plant Biotechnology Laboratory, Chinese Academy of Sciences, People's Republic of China.
Genome. 2000 Feb;43(1):181-4.
Two clones of a new family of tandemly repeated DNA sequences have been isolated from a maize random genomic DNA library. MR68 is 410 bp, representing a monomeric unit and MR77 is 1222 bp, containing three units. The copy number was estimated to be about 3000 per 1C maize genome. Its methylation pattern was also determined. Fluorescent in situ hybridization (FISH) indicates that the sequence is located on the subtelomeric region of the long arm of chromosomes 3 and 6, as well as on the satellite of chromosome 6.
从玉米随机基因组DNA文库中分离出了一个新的串联重复DNA序列家族的两个克隆。MR68为410 bp，代表一个单体单元；MR77为1222 bp，包含三个单元。据估计，其拷贝数约为每1C玉米基因组3000个。还确定了其甲基化模式。荧光原位杂交（FISH）表明，该序列位于3号和6号染色体长臂的亚端粒区域，以及6号染色体的随体上。
Zotero 插件