日本鳗鲡淡水和深海视杆视蛋白基因的分子克隆及性成熟过程中的表达分析

Molecular cloning of fresh water and deep-sea rod opsin genes from Japanese eel Anguilla japonica and expressional analyses during sexual maturation.

作者信息

Zhang H, Futami K, Horie N, Okamura A, Utoh T, Mikawa N, Yamada Y, Tanaka S, Okamoto N

机构信息

IRAGO Institute, Shinden 377-Ehima, Atsumi-cho, Atsumi-gun, Aichi, Japan.

出版信息

FEBS Lett. 2000 Mar 3;469(1):39-43. doi: 10.1016/s0014-5793(00)01233-3.

DOI:10.1016/s0014-5793(00)01233-3

PMID:10708752

Abstract

We have determined the complete cDNA sequences of fresh water rod opsin gene (fwo) and deep-sea rod opsin gene (dso) from Japanese eel Anguilla japonica. The cDNA clones of fwo and dso consisted of 1437 and 1497 nucleotides, respectively. The predicted opsins of both genes consisted of 352 amino acid residues. Southern blot and PCR analyses of genomic DNA indicated that the Japanese eel genome contains only one fwo and one dso and they are intronless. Quantitative RT-PCR analyses revealed that the expression of fwo decreases with sexual maturation while that of dso increases.

摘要

我们已经测定了日本鳗鲡（Anguilla japonica）淡水视杆视蛋白基因（fwo）和深海视杆视蛋白基因（dso）的完整cDNA序列。fwo和dso的cDNA克隆分别由1437和1497个核苷酸组成。两个基因预测的视蛋白均由352个氨基酸残基组成。基因组DNA的Southern印迹和PCR分析表明，日本鳗鲡基因组仅包含一个fwo和一个dso，且它们没有内含子。定量RT-PCR分析显示，fwo的表达随着性成熟而降低，而dso的表达则增加。