四种含硝基芳香胺在鼠伤寒沙门氏菌组氨酸基因中诱导的诱变特异性比较。

Comparison of the mutagenic specificity induced by four nitro-group-containing aromatic amines in Salmonella typhimurium his genes.

作者信息

Chung K, Hughes T J, Claxton L D

机构信息

Department of Microbiology and Molecular Cell Sciences, The University of Memphis, Memphis, TN 38152, USA.

出版信息

Mutat Res. 2000 Feb 16;465(1-2):165-71. doi: 10.1016/s1383-5718(99)00225-9.

DOI:10.1016/s1383-5718(99)00225-9

PMID:10708983

Abstract

Four nitrated aromatic amines (2-nitro-p-phenylenediamine [2NPD], 3-nitro-o-phenylenediamine [3NPD], 4-nitro-o-phenylenediamine [4NPD] and 4,4'-dinitro-2-biphenylamine [DNBA]) are direct-acting mutagens in Salmonella typhimurium strain TA100. These compounds were tested further using the Xenometrix strains of S. typhimurium: TA7001, TA7002, TA7003, TA7004, TA7005, and TA7006, with and without S9 mix in the plate incorporation assay. The direct-acting mutagenicity of 2NPD, 4NPD, and DNBA was detected with TA7002, TA7004 and TA7005. 2NPD and DNBA showed some activity in TA7006; DNBA also showed some activity in TA7003. Mutagenicity was generally decreased in these strains when S9 was added. 3NPD was mutagenic in TA7004 without S9 and in TA7005 with and without S9. These data suggest that 2NPD, 4NPD and DNBA induced TA-->AT and CG-->AT transversions as well as GC-->AT transitions in the his gene. 3NPD induced CG-->AT transversions and GC-->AT transitions. 2NPD and DNBA also induced a small portion of CG-->GC transversions.

摘要

四种硝化芳香胺（2-硝基对苯二胺[2NPD]、3-硝基邻苯二胺[3NPD]、4-硝基邻苯二胺[4NPD]和4,4'-二硝基-2-联苯胺[DNBA]）是鼠伤寒沙门氏菌TA100菌株中的直接作用诱变剂。使用鼠伤寒沙门氏菌的Xenometrix菌株（TA7001、TA7002、TA7003、TA7004、TA7005和TA7006）在平板掺入试验中添加或不添加S9混合物对这些化合物进行了进一步测试。在TA7002、TA7004和TA7005中检测到了2NPD、4NPD和DNBA的直接作用致突变性。2NPD和DNBA在TA7006中表现出一定活性；DNBA在TA7003中也表现出一定活性。添加S9后，这些菌株中的致突变性通常会降低。3NPD在无S9的TA7004以及有和无S9的TA7005中具有致突变性。这些数据表明，2NPD、4NPD和DNBA在his基因中诱导了TA→AT和CG→AT颠换以及GC→AT转换。3NPD诱导了CG→AT颠换和GC→AT转换。2NPD和DNBA还诱导了一小部分CG→GC颠换。