Iwata T, Inoue K, Kato T, Nishida T, Kinoshita H
Second Department of Surgery, Osaka City University Hospital, Japan.
Osaka City Med J. 1999 Dec;45(2):109-21.
TCR-gamma delta cells develop by extrathymic differentiation and might play an important role in thymectomy-resistant myasthenia gravis (MG) patients. In this study, we show development of human TCR-gamma delta cells in periphery of SCID mice by transplantation of human MG thymus or thymoma.
Three pieces of thymoma tissue and four of non-thymoma thymic tissues were obtained from MG patients by thymectomy. Each tissue was transplanted into 5-6 weeks old female SCID mice by intraperitoneal injection or surgical implantation. Rate of human TCR-positive cell development and its subtypes (TCR-alpha beta, TCR-gamma delta) were measured in the mice blood at one, three, and six weeks after the transplantation.
Human TCR-positive cells were detected three weeks after transplantation. Rate of TCR-gamma delta T cell development got higher in thymoma transplanted group than in non-thymoma transplanted group.
We could successfully develop human mature T cells in SCID mice by transplantation of human MG thymus or thymoma.
TCR-γδ细胞通过胸腺外分化发育，可能在胸腺切除术后抵抗的重症肌无力（MG）患者中发挥重要作用。在本研究中，我们通过移植人MG胸腺或胸腺瘤，展示了人TCR-γδ细胞在SCID小鼠外周的发育情况。
通过胸腺切除术从MG患者获取3块胸腺瘤组织和4块非胸腺瘤胸腺组织。将每块组织通过腹腔注射或手术植入的方式移植到5-6周龄的雌性SCID小鼠体内。在移植后1周、3周和6周测量小鼠血液中人TCR阳性细胞的发育率及其亚型（TCR-αβ、TCR-γδ）。
移植后3周检测到人TCR阳性细胞。胸腺瘤移植组中TCR-γδ T细胞的发育率高于非胸腺瘤移植组。
通过移植人MG胸腺或胸腺瘤，我们能够在SCID小鼠中成功培育出人成熟T细胞。
