通过16 - 23S间隔区扩增对木糖葡萄球菌进行核糖体分型及快速鉴定

Ribotyping and rapid identification of Staphylococcus xylosus by 16-23S spacer amplification.

作者信息

Villard L, Kodjo A, Borges E, Maurin F, Richard Y

机构信息

Ecole Nationale Vétérinaire de Lyon, 1 Avenue Bourgelat, BP 83, 69280, Marcy l'Etoile, France.

出版信息

FEMS Microbiol Lett. 2000 Apr 1;185(1):83-7. doi: 10.1111/j.1574-6968.2000.tb09044.x.

DOI:10.1111/j.1574-6968.2000.tb09044.x

PMID:10731611

Abstract

Ninety-five strains of Staphylococcus xylosus isolated from goat milk, French sausage or mice were analyzed together with 35 Staphylococcus type strains by 16-23S spacer amplification and ribotyping. The results obtained by PCR amplification of the 16-23S spacer region permitted the distinction of each type strain and additionally generated a DNA banding pattern characteristic for 93 of the 95 Staphylococcus xylosus strains. Ribotyping proved to be an efficient epidemiological tool for Staphylococcus xylosus species as it clustered the 95 strains into 23 distinct types.

摘要

对从山羊奶、法式香肠或小鼠中分离出的95株木糖葡萄球菌菌株与35株葡萄球菌标准菌株一起，通过16 - 23S间隔区扩增和核糖体分型进行分析。通过对16 - 23S间隔区进行PCR扩增得到的结果能够区分每一株标准菌株，此外还产生了95株木糖葡萄球菌菌株中93株所特有的DNA条带模式。核糖体分型被证明是木糖葡萄球菌种有效的流行病学工具，因为它将95株菌株聚类为23个不同的类型。