Miller B G, Hassell A M, Milburn M V, Short S A
Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599-7260, USA.
Acta Crystallogr D Biol Crystallogr. 2000 Apr;56(Pt 4):472-4. doi: 10.1107/s0907444900000949.
Crystals of the Saccharomyces cerevisiae pyrimidine biosynthetic enzyme orotidine 5'-phosphate decarboxylase (ODCase) were grown by the hanging-drop vapor-diffusion technique at 277 K using polyethylene glycol 4000 as the precipitant. Crystals of native and selenomethionyl ODCase diffract to less than 2.2 A and belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 90.1, b = 116.2, c = 117.0 A. Crystals of ODCase grown in the presence of the postulated transition-state analog inhibitor 6-hydroxyuridine 5'--phosphate (BMP) diffract to less than 2.5 A and belong to space group P2(1), with unit-cell parameters a = 79.9, b = 80.0, c = 98.2 A, beta = 108.6 degrees.
采用悬滴气相扩散技术，以聚乙二醇4000作为沉淀剂，在277 K条件下培养出酿酒酵母嘧啶生物合成酶乳清苷5'-磷酸脱羧酶（ODCase）的晶体。天然型和硒代蛋氨酸型ODCase晶体的衍射分辨率小于2.2 Å，属于正交晶系空间群P2(1)2(1)2(1)，晶胞参数为a = 90.1、b = 116.2、c = 117.0 Å。在假定的过渡态类似物抑制剂6-羟基尿苷5'-磷酸（BMP）存在的情况下培养出的ODCase晶体，其衍射分辨率小于2.5 Å，属于空间群P2(1)，晶胞参数为a = 79.9、b = 80.0、c = 98.2 Å，β = 108.6°。
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