肌萎缩侧索硬化症患者运动皮层和新皮层中神经元总数不变：一项体视学研究。

Unchanged total number of neurons in motor cortex and neocortex in amyotrophic lateral sclerosis: a stereological study.

作者信息

Gredal O, Pakkenberg H, Karlsborg M, Pakkenberg B

机构信息

Research Institute of Biological Psychiatry, H:S St. Hans Mental Hospital, Roskilde, Dennark.

出版信息

J Neurosci Methods. 2000 Feb 15;95(2):171-6. doi: 10.1016/s0165-0270(99)00175-2.

DOI:10.1016/s0165-0270(99)00175-2

PMID:10752488

Abstract

Modern stereological methods provide precise and reliable estimates of the number of neurons in specific regions of the brain. The total number of neurons in the neocortex and motor cortex from eight patients suffering from amyotrophic lateral sclerosis (ALS) and nine controls was estimated. No attempt was made to estimate subpopulations of neurons such as the number of giant pyramidal cells of Betz. No difference was found in the average number of neurons in neocortex in ALS and controls, 21.7 and 22.3 x 10(9), respectively, and 1.33 and 1.29 x 10(9) in motor cortex, respectively. In the light of our stereological measurements, results obtained from in-vivo proton magnetic resonance spectroscopy (1H-MRS), suggesting neuronal loss in ALS, may instead be due to neuronal metabolic dysfunction and/or alteration in the size or the volume fraction of the neurons.

摘要

现代体视学方法能对大脑特定区域的神经元数量进行精确且可靠的估计。我们估计了8例肌萎缩侧索硬化症（ALS）患者和9例对照者新皮层和运动皮层中的神经元总数。未尝试估计神经元亚群数量，如贝茨巨锥体细胞的数量。ALS患者和对照者新皮层中神经元的平均数量无差异，分别为21.7和22.3×10⁹，运动皮层中分别为1.33和1.29×10⁹。根据我们的体视学测量结果，体内质子磁共振波谱（¹H-MRS）得出的提示ALS患者存在神经元丢失的结果，可能反而归因于神经元代谢功能障碍和/或神经元大小或体积分数的改变。