褶曲酰胺：通向膜色素生物合成起源的线索？

Plicatamide: A lead to the biosynthetic origins of the tunichromes?

作者信息

Tincu J A, Craig A G, Taylor S W

机构信息

Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California, San Diego, La Jolla, California 92093-0204, USA.

出版信息

Biochem Biophys Res Commun. 2000 Apr 13;270(2):421-4. doi: 10.1006/bbrc.2000.2449.

DOI:10.1006/bbrc.2000.2449

PMID:10753640

Abstract

Plicatamide is a modified octapeptide from the ascidian Styela plicata having the structure Phe-Phe-His-Leu-His-Phe-His-decarboxyDeltaDOPA (where decarboxyDeltaDOPA = decarboxy-(E)-alpha,beta-dehydro-3, 4-dihydroxyphenylalanine). Edman sequencing, tandem mass spectrometry, and proton NMR have been used to characterize 100 microg of the compound. Plicatamide represents an important link between two classes of biomolecules: the tunichromes which share an oxidatively decarboxylated C-terminus and higher molecular weight DOPA-polypeptides. The 8-residue sequence provides the first opportunity to investigate the biosynthetic origins of the tunichrome family by molecular biological techniques.

摘要

褶牡蛎酰胺是一种来自皱瘤海鞘的修饰八肽，其结构为苯丙氨酸-苯丙氨酸-组氨酸-亮氨酸-组氨酸-苯丙氨酸-组氨酸-脱羧δ-DOPA（其中脱羧δ-DOPA = 脱羧-（E）-α，β-脱氢-3,4-二羟基苯丙氨酸）。已使用埃德曼测序、串联质谱和质子核磁共振对100微克该化合物进行了表征。褶牡蛎酰胺代表了两类生物分子之间的重要联系：具有氧化脱羧C末端的被囊色素和更高分子量的多巴多肽。这个8个残基的序列为通过分子生物学技术研究被囊色素家族的生物合成起源提供了首个机会。