Pax-8、p53和bcl-2在人类甲状腺良恶性疾病中的表达

Expression of Pax-8, p53 and bcl-2 in human benign and malignant thyroid diseases.

作者信息

Puglisi F, Cesselli D, Damante G, Pellizzari L, Beltrami C A, Di Loreto C

机构信息

Institute of Clinical Oncology, University of Udine, Italy.

出版信息

Anticancer Res. 2000 Jan-Feb;20(1A):311-6.

PMID:10769673

Abstract

BACKGROUND

Immunohistochemical expression of the transcription factor Pax-8 in human thyroid diseases has never been investigated. The relationship between Pax-8, bcl-2 and p53 in thyroid neoplasms is also matter of interest.

MATERIALS AND METHODS

Seventy-three thyroid tissue samples were evaluated for the expression of Pax-8, p53 and bcl-2 using the immunoperoxidase technique. The series included 11 follicular adenomas, 11 goitres, 23 papillary carcinomas, 16 follicular carcinomas, 6 undifferentiated carcinomas and 6 medullary carcinomas.

RESULTS

The percentage of Pax-8 positive cells ranged from 14.9 to 27.1% and 10.1 to 39% in goitres and follicular adenomas, respectively. Among differentiated carcinomas, follicular histotype showed a Pax-8 immunoreactivity ranging from 0 to 26.5% of the neoplastic cells whereas in papillary carcinomas the percentage of positive cells ranged from 0 to 16.8%. None out of the six undifferentiated carcinomas showed Pax-8 immunoreactivity. The same negative pattern was noticed in medullary carcinomas. A statistically significant difference in Pax-8 expression was observed between non-malignant and malignant diseases (p < 0.0001). A different reactivity for Pax-8 was also noticed between differentiated carcinomas and undifferentiated carcinomas (p = 0.07). None of the benign tissues stained for p53 whereas among malignant specimens different percentages of p53 expression were observed with all undifferentiated carcinomas expressing the highest positivity (range 24.1-88.6%). Finally, when a combined analysis of bcl-2 and Pax-8 reactivity was carried out, some carcinomas proved to be Pax-8 negative and bcl-2 positive whereas others showed a similar immunoreactive pattern for both Pax-8 and bcl-2.

CONCLUSIONS

Pax-8 is mainly expressed in benign rather than in malignant thyroid diseases and, among neoplasms, differentiated carcinomas express Pax-8 more frequently than undifferentiated carcinomas. An inverse pattern was observed for p53. Bcl-2 seems to be partially related to Pax-8 expression. However, a Pax-8 independent bcl-2 expression is also evident.

摘要

背景

转录因子Pax - 8在人类甲状腺疾病中的免疫组化表达从未被研究过。甲状腺肿瘤中Pax - 8、bcl - 2和p53之间的关系也备受关注。

材料与方法

采用免疫过氧化物酶技术对73份甲状腺组织样本进行Pax - 8、p53和bcl - 2表达情况的评估。该系列样本包括11例滤泡性腺瘤、11例甲状腺肿、23例乳头状癌、16例滤泡状癌、6例未分化癌和6例髓样癌。

结果

甲状腺肿和滤泡性腺瘤中Pax - 8阳性细胞百分比分别为14.9%至27.1%和10.1%至39%。在分化型癌中，滤泡组织学类型的肿瘤细胞中Pax - 8免疫反应性为0至26.5%，而在乳头状癌中阳性细胞百分比为0至16.8%。6例未分化癌中无一例显示Pax - 8免疫反应性。髓样癌中也观察到相同的阴性模式。在非恶性和恶性疾病之间观察到Pax - 8表达存在统计学显著差异（p < 0.0001）。在分化型癌和未分化癌之间也注意到Pax - 8的不同反应性（p = 0.07）。良性组织中无p53染色，而在恶性样本中观察到不同百分比的p53表达，所有未分化癌的阳性率最高（范围为24.1% - 88.6%）。最后，当对bcl - 2和Pax - 8反应性进行联合分析时，一些癌被证明为Pax - 8阴性和bcl - 2阳性，而其他一些则显示Pax - 8和bcl - 2具有相似的免疫反应模式。