酪蛋白激酶II对105千道尔顿热休克蛋白HSP105α和HSP105β的磷酸化作用。
Phosphorylation of the 105-kDa heat shock proteins, HSP105alpha and HSP105beta, by casein kinase II.
作者信息
Ishihara K, Yasuda K, Hatayama T
机构信息
Department of Biochemistry, Kyoto Pharmaceutical University, 5 Nakauchicho, Misasagi, Yamashina-ku, Kyoto, 607-8414, Japan.
出版信息
Biochem Biophys Res Commun. 2000 Apr 21;270(3):927-31. doi: 10.1006/bbrc.2000.2541.
The 105-kDa heat shock protein alpha (HSP105alpha) and HSP105beta are mammalian heat shock proteins that belong to the HSP105/HSP110 family. Both HSP105alpha and HSP105beta consist of acidic and basic isoforms. Here we report that the acidic isoforms are serine phosphorylated HSP105alpha or HSP105beta. Furthermore, using an in-gel kinase assay with HSP105alpha or HSP105beta as the substrate, the protein kinase that phosphorylates HSP105alpha and HSP105beta was identified as casein kinase II. Since phosphorylated HSP105alpha is especially prominent in the brain compared to other tissues of mice and rats, the phosphorylation of HSP105alpha by casein kinase II may be biologically significant.
105千道尔顿热休克蛋白α（HSP105α）和HSP105β是属于HSP105/HSP110家族的哺乳动物热休克蛋白。HSP105α和HSP105β均由酸性和碱性亚型组成。在此我们报告，酸性亚型是丝氨酸磷酸化的HSP105α或HSP105β。此外，使用以HSP105α或HSP105β为底物的凝胶内激酶测定法，将使HSP105α和HSP105β磷酸化的蛋白激酶鉴定为酪蛋白激酶II。由于与小鼠和大鼠的其他组织相比，磷酸化的HSP105α在脑中尤为突出，酪蛋白激酶II对HSP105α的磷酸化可能具有生物学意义。
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