Vandenbol M, Fairhead C
Unité de Microbiologie, Faculté Universitaire des Sciences Agronomiques de Gembloux, 6 avenue Maréchal Juin, B-5030, Gembloux, Belgium. vandenbol.m.fsagx.ac.be
Gene. 2000 Apr 18;247(1-2):45-52. doi: 10.1016/s0378-1119(00)00127-x.
Nineteen open reading frames (ORFs) in the left arm of chromosome XI of the yeast Saccharomyces cerevisiae were inactivated. This was done by producing single-gene or contiguous-gene deletions in haploid and diploid strains. Four deletions are lethal to the corresponding haploid strains, and two result in a failure to grow on a rich glycerol medium. Complementation experiments showed that five of the six identified phenotypes were due to deletion of a single gene (ORFs YKL173w, YKL172w, YKL165c, YKL154w are essential, and YKL160w is required for growth on glycerol medium). One of the phenotypes observed on glycerol medium was not suppressed by the corresponding deleted genes. None of the other deletions, covering 13 ORFs in all, gave rise to any obvious phenotype when the cells were grown at three different temperatures on rich glycerol or glucose medium or on minimal synthetic medium.
酿酒酵母（Saccharomyces cerevisiae）第XI号染色体左臂上的19个开放阅读框（ORF）被失活。这是通过在单倍体和二倍体菌株中产生单基因或连续基因缺失来实现的。四个缺失对相应的单倍体菌株是致死的，两个导致在丰富甘油培养基上无法生长。互补实验表明，六个已鉴定表型中的五个是由于单个基因的缺失（ORF YKL173w、YKL172w、YKL165c、YKL154w是必需的，YKL160w是在甘油培养基上生长所必需的）。在甘油培养基上观察到的一种表型未被相应的缺失基因抑制。当细胞在丰富甘油或葡萄糖培养基或基本合成培养基上的三种不同温度下生长时，其他所有覆盖13个ORF的缺失均未产生任何明显表型。
