猪3型腺病毒E1区、pIX和pIVa2基因以及两个新开放阅读框的序列分析

Sequence analysis of porcine adenovirus type 3 E1 region, pIX and pIVa2 genes, and two novel open reading frames.

作者信息

Aggarwal N, Mittal S K

机构信息

Department of Veterinary Pathobiology, School of Veterinary Medicine, Purdue University, West Lafayette, Ind., USA.

出版信息

Intervirology. 2000;43(1):6-12. doi: 10.1159/000025016.

DOI:10.1159/000025016

PMID:10773731

Abstract

The porcine adenovirus type 3 (PAd3) genome between map units 0 and 13.7 was sequenced and compared with similar regions of other adenoviruses. This region consists of the left inverted terminal repeat sequences involved in DNA packaging, the entire early region 1 (E1) and the protein IX (pIX) transcription unit. The lower strand contains the C-terminal end of IVa2 of the E2A transcriptional unit and two novel open reading frames (ORFs). The E1 transcription unit consists of ORFs for proteins homologous to the E1A, E1B-17k and E1B-55k of both human adenovirus type 5 (HAd5) and bovine adenovirus type 3 (BAd3). The predicted PAd3 pIX demonstrated homology with the N-terminal portion of the pIXs of HAd5 and BAd3. On the lower strand, immediately after the putative IVa2 ORF, there are two unique ORFs of 208 and 203 amino acid residues that showed homology with Epstein-Barr virus nuclear antigens and other cellular transcription factors.

摘要

对猪3型腺病毒（PAd3）基因组中位于图谱单位0至13.7之间的区域进行了测序，并与其他腺病毒的相似区域进行了比较。该区域由参与DNA包装的左侧反向末端重复序列、整个早期区域1（E1）和蛋白质IX（pIX）转录单位组成。下链包含E2A转录单位IVa2的C末端以及两个新的开放阅读框（ORF）。E1转录单位由与人类5型腺病毒（HAd5）和牛3型腺病毒（BAd3）的E1A、E1B - 17k和E1B - 55k同源的蛋白质的ORF组成。预测的PAd3 pIX与HAd5和BAd3的pIX的N末端部分具有同源性。在下链上，紧接假定的IVa2 ORF之后，有两个分别含208和203个氨基酸残基的独特ORF，它们与爱泼斯坦 - 巴尔病毒核抗原及其他细胞转录因子具有同源性。