Hashiguchi K, Hashimoto K
Department of Oral Surgery, School of Medicine, Hamamatsu University, Shizuoka, Japan.
Okajimas Folia Anat Jpn. 2000 Mar;76(6):321-33. doi: 10.2535/ofaj1936.76.6_321.
Spectra of human dental enamel was recorded in the 200-400 nm UV region. It showed the weak band at 280 nm which were present in enamel protein. Excimer laser are gas lasers which emit light with photochemical decomposition. The wavelength depends on the 248 nm with krypton-fluoride. The enamel surfaces of extracted human teeth were exposed to KrF excimer laser by an energy density range from 29.6 to 3200 J/cm2. The purpose of this study was to investigate the changes with photo chemical reaction in enamel by light microscopy, SEM and X ray diffraction method. Results of analyses suggested that the observed changes of enamel exposed to this laser were the alpha and beta-tricalcium phosphate (TCP) phase in small amounts. No histological changes were observed in grain boundaries of cross sectioned lased enamel under light microscopy. The SEM examination revealed a roughened surface with bubble formation at 800-3200 J/cm2. SEM of etched enamel surface with 0.1 N HCl after laser irradiation at 400-800 J/cm2 showed the extension along the length of the rods. At 1600-3200 J/cm2, there appeared to be a melting of prism structures, because of conversion of photon energy into thermal energy. These results showed the KrF excimer laser irradiation to dental enamel might be a new type of treatment modality and diagnosis in preventive dentistry.
在200-400纳米的紫外区域记录了人类牙釉质的光谱。它显示出在280纳米处存在于牙釉质蛋白中的弱带。准分子激光是一种气体激光,它通过光化学分解发射光。波长取决于氪氟化物的248纳米。将拔除的人类牙齿的牙釉质表面暴露于能量密度范围为29.6至3200 J/cm²的KrF准分子激光下。本研究的目的是通过光学显微镜、扫描电子显微镜和X射线衍射方法研究牙釉质光化学反应的变化。分析结果表明,观察到的暴露于该激光的牙釉质变化是少量的α和β-磷酸三钙(TCP)相。在光学显微镜下,未观察到激光照射牙釉质横截面晶界的组织学变化。扫描电子显微镜检查显示,在800-3200 J/cm²时表面粗糙并形成气泡。在400-800 J/cm²激光照射后,用0.1 N HCl蚀刻牙釉质表面的扫描电子显微镜显示沿牙釉柱长度的延伸。在1600-3200 J/cm²时,由于光子能量转化为热能,似乎出现了棱柱结构的熔化。这些结果表明,KrF准分子激光照射牙釉质可能是预防牙科学中的一种新型治疗方式和诊断方法。