Strózycki P M, Karłowski W M, Dessaux Y, Petit A, Legocki A B
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan.
Mol Gen Genet. 2000 Mar;263(2):173-82. doi: 10.1007/s004380051158.
The proximal parts of the promoters of the genes for symbiotic-type hemoglobins are generally conserved, but the promoter of the lbI gene of lupine (LulbI) shows some unusual structural features. It lacks typical organ-specific elements characteristic of all the leghemoglobin gene promoters described thus far. We have analysed its functional activity in transgenic Lotus corniculatus. A fusion construct between the lbI promoter and the GUS reporter gene was expressed mainly in the central zone of the root nodule, but the product was also detected in the non-nodule root zone and in roots in tissue culture. In roots of transgenic tobacco, the activity of the promoter was only 24% lower than in Lotus nodules. LulbI promoter activity was also detected in tobacco leaves. Lupine hemoglobin I has a higher sequence identity to symbiotic-type hemoglobins and thus it groups within the "Class II" hemoglobins.
共生型血红蛋白基因启动子的近端部分通常是保守的，但羽扇豆（LulbI）的lbI基因启动子表现出一些不寻常的结构特征。它缺乏迄今为止所描述的所有豆血红蛋白基因启动子特有的典型器官特异性元件。我们已经分析了其在转基因百脉根中的功能活性。lbI启动子与GUS报告基因之间的融合构建体主要在根瘤的中央区域表达，但在非根瘤根区和组织培养的根中也检测到了产物。在转基因烟草的根中，启动子的活性仅比百脉根根瘤中的活性低24%。在烟草叶片中也检测到了LulbI启动子活性。羽扇豆血红蛋白I与共生型血红蛋白具有更高的序列同一性，因此它属于“II类”血红蛋白。
