聚合酶链反应在结核病诊断中的应用

Polymerase chain reaction in the diagnosis of tuberculosis.

作者信息

Jatana S K, Nair M N, Lahiri K K, Sarin N P

机构信息

Department of Pediatrics and Command Pathology Laboratory, Central Command, Lucknow Cantonment 226 002, India.

出版信息

Indian Pediatr. 2000 Apr;37(4):375-82.

PMID:10781230

Abstract

OBJECTIVES

To evaluate efficacy of polymerase chain reaction (PCR), using the insertion sequence IS6110 as target for DNA, to detect Mycobacterium tuberculosis in body fluids of children with suspected tuberculosis (TB).

SETTING

Hospitalized patients.

METHODS

A comparison of PCR on body fluids, Acid Fast Bacilli staining (AFB), mycobacterial culture and clinical features, with special emphasis on central nervous system (CNS) TB was done over 18 month period. A total of 80 children were evaluated, 41 with probable TB disease and 39 controls. Cases were defined by specific clinical criteria. Controls included patients free of clinical TB. PCR was done on the clinical specimens and compared with clinical findings, radiological features, Mantoux (Mx) testing, AFB staining and culture on Lowenstein-Jensen (LJ) medium.

RESULTS

Sensitivity of PCR in CSF samples was 100%, in gastric aspirate samples was 20% and in pleural fluid samples was 100%.

CONCLUSION

PCR technique may become a valuable diagnostic tool for the diagnosis of tuberculosis in children especially in CNS TB.

摘要

目的

评估以插入序列IS6110作为DNA靶点的聚合酶链反应（PCR）检测疑似结核病（TB）儿童体液中结核分枝杆菌的效能。

研究地点

住院患者。

方法

在18个月的时间里，对体液PCR、抗酸杆菌染色（AFB）、分枝杆菌培养及临床特征进行比较，特别关注中枢神经系统（CNS）结核。共评估了80名儿童，其中41名可能患有结核病，39名作为对照。病例根据特定临床标准定义。对照包括无临床结核病的患者。对临床标本进行PCR检测，并与临床发现、放射学特征、结核菌素试验（Mx）、AFB染色及罗氏（LJ）培养基培养结果进行比较。