急性白血病患儿光动力治疗清除骨髓：氨磷汀的细胞保护作用

Bone marrow purging by photodynamic treatment in children with acute leukemia: cytoprotective action of amifostine.

作者信息

Danilatou V, Lydaki E, Dimitriou H, Papazoglou T, Kalmanti M

机构信息

Department of Pediatric Hematology/Oncology, University Hospital of Heraklion, Foundation for Research and Technology, Laser and Applications Division, University of Crete Medical School, 71110, Heraklion, Greece.

出版信息

Leuk Res. 2000 May;24(5):427-35. doi: 10.1016/s0145-2126(99)00202-7.

DOI:10.1016/s0145-2126(99)00202-7

PMID:10785265

Abstract

In order to evaluate the combined effect of Amifostine and Merocyanine 540 during photoirradiation in neoplastic cells, bone marrow cells from children with acute leukemia (AL), age-matched controls as well as HL-60 cell line were studied. Cell suspensions were incubated with Amifostine, then with MC 540 and they were subsequently exposed to different irradiation doses by Argon Laser 514 nm. Cell survival was estimated by trypan blue supravital stain following a 24-h incubation. The leukemic cell line was studied in continuous liquid cell cultures for 4 weeks. The survival of normal bone marrow progenitors has been estimated by colony formation assay in methylcellulose cultures. Our results showed that Amifostine enhances the photokilling effect of MC 540 on leukemic cells and significantly protects bone marrow nucleated and committed progenitors (BFU-E and CFU-GM) from children with AL under chemotherapy. In conclusion, Amifostine seems to be a promising cytoprotective agent in the clinical use of purging with MC 540 mediated phototherapy.

摘要

为了评估氨磷汀和美菁540在肿瘤细胞光照射过程中的联合作用，对急性白血病（AL）患儿的骨髓细胞、年龄匹配的对照者以及HL-60细胞系进行了研究。细胞悬液先用氨磷汀孵育，然后用美菁540孵育，随后用514nm氩激光照射不同剂量。孵育24小时后，通过台盼蓝活体染色评估细胞存活率。对白血病细胞系在连续液体细胞培养中进行了4周的研究。通过甲基纤维素培养中的集落形成试验评估正常骨髓祖细胞的存活率。我们的结果表明，氨磷汀增强了美菁540对白血病细胞的光杀伤作用，并显著保护接受化疗的急性白血病患儿的骨髓有核祖细胞和定向祖细胞（BFU-E和CFU-GM）。总之，在美菁540介导的光疗净化临床应用中，氨磷汀似乎是一种有前景的细胞保护剂。