Lucau-Danila A, Wysocki R, Roganti T, Foury F
Unité de Biochimie Physiologique, Place Croix-du-Sud 2-20, B-1348 Louvain-la-Neuve, Belgium.
Yeast. 2000 Apr;16(6):547-52. doi: 10.1002/(SICI)1097-0061(200004)16:6<547::AID-YEA552>3.0.CO;2-2.
In the framework of the European Network for Functional Analysis (EUROFAN), five packages of 96 ORFs from chromosomes III, IV, VII, XIII, XIV and XV were subjected to systematic deletions in an isogenic derivative of strain S288c. Deletions were constructed in diploid and haploid strains. Two questionable ORFs overlapping with larger ORFs and seven TY ORFs were discarded. A total of 456 heterozygous and 385 homozygous deletant diploids were obtained. Sixty-nine deletions, 25 of which had never been published before, were lethal in haploid strains and 30 caused slow cellular growth.
在欧洲功能分析网络（EUROFAN）的框架下，对来自III、IV、VII、XIII、XIV和XV号染色体的5组各96个开放阅读框（ORF）在S288c菌株的同基因衍生物中进行了系统缺失。缺失构建于二倍体和单倍体菌株中。两个与较大开放阅读框重叠的可疑开放阅读框和七个TY开放阅读框被舍弃。共获得456个杂合缺失二倍体和385个纯合缺失二倍体。69个缺失（其中25个此前从未发表过）在单倍体菌株中是致死的，30个缺失导致细胞生长缓慢。
