Kim W H, Lee J H, Han S U, Jin Y M, Kwak Y S, Wang H J, Kim M W
Department of Surgery, Biochemistry, Ajou University School of Medicine, Suwon, Korea.
Hepatogastroenterology. 2000 Mar-Apr;47(32):371-4.
BACKGROUND/AIMS: Acute liver failure either after liver resection or as part of underlying liver disease is still associated with high mortality. Hepatocyte transplantation in various forms has attracted attention recently. However, none of those reports have investigated the thorough and systematic analysis of effect of hepatocyte transplantation on acute liver failure induced by 90% hepatectomy. Therefore, we investigated systematic analysis of effect of hepatocyte transplantation on rats with acute liver failure.
Male Sprague-Dawley rats were used. Group I rats (n = 26) received intrasplenic injection of 2 x 10(7) hepatocytes in 0.3 mL Dulbecco's modified Eagle's medium (DMEM) 24 hours prior to 90% hepatectomy. Group II rats (n = 24) received intrasplenic injection of DMEM only. Twenty-two rats from group I and 20 from group II were observed for the determination of survival time. The remaining 8 (4/each group) rats were used to assess the liver function and regeneration.
The hepatocyte bearing spleen revealed active glucose-6-phosphatase activity. In group I rats, the survival was longer and that group had more long-term survivors than those of group II controls. In group I, there was significant increase in the ratio of weight of remnant liver lobes to body weight. At 24 hours after hepatectomy, group I rats had improved biochemical parameters compared to those of group II rats.
In rats with acute liver failure, intrasplenic hepatocyte transplantation acts as a bridge to support experimental rats from acute liver failure to liver regeneration, prolong survival in rats with acute liver failure and improve biochemical parameters.
背景/目的：肝切除术后或作为潜在肝脏疾病一部分的急性肝衰竭，其死亡率仍然很高。各种形式的肝细胞移植最近引起了关注。然而，这些报告均未对肝细胞移植对90%肝切除诱导的急性肝衰竭的影响进行全面系统的分析。因此，我们对肝细胞移植对急性肝衰竭大鼠的影响进行了系统分析。
使用雄性Sprague-Dawley大鼠。I组大鼠（n = 26）在90%肝切除术前24小时经脾内注射0.3 mL Dulbecco改良Eagle培养基（DMEM）中的2×10⁷个肝细胞。II组大鼠（n = 24）仅经脾内注射DMEM。观察I组的22只大鼠和II组的20只大鼠的生存时间。其余8只（每组4只）大鼠用于评估肝功能和肝再生。
携带肝细胞的脾脏显示出活跃的葡萄糖-6-磷酸酶活性。I组大鼠的生存期更长，长期存活者比II组对照组更多。在I组中，残余肝叶重量与体重的比值显著增加。肝切除术后24小时，I组大鼠的生化参数比II组大鼠有所改善。
在急性肝衰竭大鼠中，经脾内肝细胞移植可作为一种桥梁，支持实验大鼠从急性肝衰竭过渡到肝再生，延长急性肝衰竭大鼠的生存期并改善生化参数。
