氯贝丁酯对大鼠白色和棕色脂肪组织中乙酰辅酶A羧化酶mRNA水平的差异作用。

Differential effect of clofibrate on acetyl-CoA carboxylase mRNA level in rat white and brown adipose tissue.

作者信息

Karbowska J, Kochan Z, Swierczyński J

机构信息

Department of Biochemistry, Medical University of Gdansk, Poland.

出版信息

Life Sci. 2000;66(6):545-52. doi: 10.1016/s0024-3205(99)00624-4.

DOI:10.1016/s0024-3205(99)00624-4

PMID:10794071

Abstract

Regulation of some lipogenic enzyme gene expression by clofibrate was studied in rat white and brown adipose tissue. In white adipose tissue the drug administration for 14 days to rats resulted in the increase in acetyl-CoA carboxylase, ATP-citrate lyase, and glucose 6-phosphate dehydrogenase mRNA levels. Opposing effect of clofibrate on the acetyl-CoA carboxylase, ATP-citrate lyase, and glucose 6-phosphate dehydrogenase mRNA levels was found in brown adipose tissue. These data indicate a tissue specificity of clofibrate action on lipogenic enzyme gene expression. The results presented in this paper provide further evidence that hypolipidaemia caused by the treatment with clofibrate cannot be related to the inhibition of fatty acid synthesis in white adipose tissue in rat.

摘要

研究了氯贝丁酯对大鼠白色和棕色脂肪组织中某些生脂酶基因表达的调控作用。给大鼠连续14天给药该药物后，白色脂肪组织中乙酰辅酶A羧化酶、ATP-柠檬酸裂解酶和葡萄糖6-磷酸脱氢酶的mRNA水平升高。而在棕色脂肪组织中，氯贝丁酯对乙酰辅酶A羧化酶、ATP-柠檬酸裂解酶和葡萄糖6-磷酸脱氢酶的mRNA水平产生相反的作用。这些数据表明氯贝丁酯对生脂酶基因表达的作用具有组织特异性。本文给出的结果进一步证明，氯贝丁酯治疗引起的降血脂作用与大鼠白色脂肪组织中脂肪酸合成的抑制无关。