Bauer P, Lush C W, Kvietys P R, Russell J M, Granger D N
Department of Molecular and Cellular Physiology, Louisiana State University Health Sciences Center, Shreveport, Louisiana 71130-3932, USA.
Am J Physiol Regul Integr Comp Physiol. 2000 May;278(5):R1140-7. doi: 10.1152/ajpregu.2000.278.5.R1140.
The objectives of this study were to determine 1) the changes in endothelial cell adhesion molecule expression that occur in a clinically relevant model of sepsis and 2) the dependence of these changes on endotoxin [lipopolysaccharide (LPS)]. The dual radiolabeled monoclonal antibody technique was used to quantify the expression of E- and P-selectin in LPS-sensitive (C3HeB/FeJ) and LPS-insensitive (C3H/HeJ) mice that were subjected to acute peritonitis by cecal ligation and perforation (CLP). At 6 h after CLP, the expression of both E- and P-selectin was increased in the gut (mesentery, pancreas, and small and large bowel) compared with the sham-operated and/or control animals, with a more marked response noted in LPS-insensitive mice. The lung also exhibited an increased P-selectin expression in both mouse strains. An accumulation of granulocytes, assessed using tissue myeloperoxidase activity, was noted in the lung and intestine of LPS-sensitive but not LPS-insensitive mice exposed to CLP. These results indicate that the CLP model of sepsis is associated with an upregulation of endothelial selectins in the gut vasculature and that enteric LPS does not contribute to this endothelial cell activation response.
1）在临床相关的脓毒症模型中发生的内皮细胞黏附分子表达的变化，以及2）这些变化对内毒素[脂多糖（LPS）]的依赖性。采用双放射性标记单克隆抗体技术，对通过盲肠结扎和穿孔（CLP）导致急性腹膜炎的LPS敏感（C3HeB/FeJ）和LPS不敏感（C3H/HeJ）小鼠中E-选择素和P-选择素的表达进行定量。CLP后6小时，与假手术和/或对照动物相比，肠道（肠系膜、胰腺以及小肠和大肠）中E-选择素和P-选择素的表达均增加，LPS不敏感小鼠的反应更为明显。在两种小鼠品系的肺中，P-选择素表达也增加。通过组织髓过氧化物酶活性评估发现，暴露于CLP的LPS敏感小鼠而非LPS不敏感小鼠的肺和肠道中有粒细胞聚集。这些结果表明，脓毒症的CLP模型与肠道血管内皮选择素上调有关，且肠道LPS对这种内皮细胞激活反应无作用。
