Alevy Y G, Battisto J R
Immunology. 1976 Mar;30(3):391-9.
Dextran given to mice caused splenic T cells to elaborate factors in vitro which heightened normal splenic B- and T-cell responses to the mitogens LPS and Con A, respectively. Chromatographic separation of dextran-triggered spleen cell supernatants revealed two T cell-derived enhancing factors which affect T cells (TDEF-TI and TDEF-TII) and two that alter B cells (TDEF-BI and TDEF-BII). All appear to be proteinaceous because exposure to trypsin destroyed their activities. Furthermore, their presence was found to be dependent upon protein synthesis since cycloheximide treatment of the cells inhibited synthesis whereas mitomycin C treatment did not. Based on absorption studies, receptors for TDEF-TI and TII were detected on thymic cells as well as on O-deficient bone marrow cells, whereas receptors for TDEF-BI and BII were on bone marrow cells but not thymic cells.
给小鼠注射葡聚糖会使脾脏T细胞在体外产生一些因子，这些因子分别增强了正常脾脏B细胞和T细胞对有丝分裂原脂多糖（LPS）和刀豆蛋白A（Con A）的反应。对葡聚糖触发的脾细胞上清液进行色谱分离，发现了两种来源于T细胞的影响T细胞的增强因子（TDEF-TI和TDEF-TII）以及两种改变B细胞的增强因子（TDEF-BI和TDEF-BII）。所有这些因子似乎都是蛋白质类的，因为用胰蛋白酶处理会破坏它们的活性。此外，发现它们的存在依赖于蛋白质合成，因为用放线菌酮处理细胞会抑制合成，而用丝裂霉素C处理则不会。基于吸收研究，在胸腺细胞以及O型缺陷骨髓细胞上检测到了TDEF-TI和TII的受体，而TDEF-BI和BII的受体则存在于骨髓细胞上，胸腺细胞上没有。
