利用扫描电子显微镜和扫描透射电子显微镜对培养的成肌C2C12细胞进行电子探针X射线微分析。

Electron probe X-ray microanalysis of cultured myogenic C2C12 cells with scanning and scanning transmission electron microscopy.

作者信息

Tylko G, Karasiński J, Wróblewski R, Roomans G M, Kilarski W M

机构信息

Department of Cytology and Histology, Jagiellonian University, Kraków, Poland.

出版信息

Folia Histochem Cytobiol. 2000;38(2):79-84.

PMID:10833672

Abstract

Heterogeneity of the elemental content of myogenic C2C12 cultured cells was studied by electron probe X-ray microanalysis (EPXMA) with scanning (SEM EPXMA) and scanning transmission electron microscopy (STEM EPXMA). The best plastic substrate for growing cells was Thermanox. For STEM EPXMA, a Formvar film coated with carbon was found to be suitable substrate. The cells examined by scanning transmission electron microscopy showed great heterogeneity in their elemental content in comparison with the cells examined in the scanning electron microscope despite of an almost identical preparation procedure for EPXMA. Nevertheless the K/Na ratios obtained from both methods of EPXMA were very close (4.1 and 4.3). We conclude that the observed discrepancy in the elemental content obtained by the two methods may be due to differences in instrumentation and this must be taken into account when planning a comparative study.

摘要

采用扫描电子显微镜电子探针X射线微分析（SEM EPXMA）和扫描透射电子显微镜电子探针X射线微分析（STEM EPXMA）研究了成肌C2C12培养细胞元素含量的异质性。生长细胞的最佳塑料基质是聚对苯二甲酸乙二酯薄膜。对于STEM EPXMA，发现涂有碳的福尔马膜是合适的基质。尽管EPXMA的制备过程几乎相同，但与扫描电子显微镜检查的细胞相比，扫描透射电子显微镜检查的细胞在元素含量上表现出很大的异质性。然而，两种EPXMA方法获得的K/Na比值非常接近（4.1和4.3）。我们得出结论，两种方法获得的元素含量存在差异可能是由于仪器不同，在规划比较研究时必须考虑到这一点。