Cattori V, Hagenbuch B, Hagenbuch N, Stieger B, Ha R, Winterhalter K E, Meier P J
Division of Clinical Pharmacology and Toxicology, Department of Medicine, University Hospital Zurich, Switzerland.
FEBS Lett. 2000 Jun 2;474(2-3):242-5. doi: 10.1016/s0014-5793(00)01596-9.
A novel organic anion transporting polypeptide (Oatp)4(1) was isolated from rat liver that is 35 amino acids longer than the reported rat liver specific organic anion transporter (rlst)-1 and exhibits a 64% amino acid sequence identity with the human OATP-C (LST-1/OATP2; gene symbol SLC21A6). When expressed in Xenopus laevis oocytes, Oatp4 (Slc21a10) mediated polyspecific uptake of a variety of organic anions including taurocholate (K(m) approximately 27 microM), bromosulfophthalein (K(m) approximately 1.1 microM) and steroid conjugates. Based on nuclease protection analysis Oatp4 appears to be the predominant transcript in rat liver indicating that rlst-1 plays a minor role in overall hepatic organic anion uptake.
从大鼠肝脏中分离出一种新型有机阴离子转运多肽（Oatp）4（1），它比已报道的大鼠肝脏特异性有机阴离子转运体（rlst）-1长35个氨基酸，并且与人类OATP-C（LST-1/OATP2；基因符号SLC21A6）具有64%的氨基酸序列同一性。当在非洲爪蟾卵母细胞中表达时，Oatp4（Slc21a10）介导多种有机阴离子的多特异性摄取，包括牛磺胆酸盐（Km约为27 microM）、溴磺酞（Km约为1.1 microM）和类固醇结合物。基于核酸酶保护分析，Oatp4似乎是大鼠肝脏中的主要转录本，这表明rlst-1在肝脏整体有机阴离子摄取中起次要作用。
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