1995/1996年南非发生非洲马瘟疫情期间，比勒陀利亚附近翁德斯特普特地区不同非洲马瘟病毒血清型的流行情况。

The prevalence of different African horsesickness virus serotypes in the Onderstepoort area near Pretoria, during an outbreak of African horsesickness in South Africa in 1995/1996.

作者信息

Bremer C W, Gerdes G H, Aitchison H, Louw I, Greyling R R, Welgemoed J

机构信息

Onderstepoort Veterinary Institute, South Africa.

出版信息

Onderstepoort J Vet Res. 2000 Mar;67(1):65-70.

PMID:10843324

Abstract

During 1995/1996 parts of South Africa experienced exceptionally high rainfall. Large numbers of Culicoides midges were seen and an outbreak of African horsesickness (AHS) followed. In the Onderstepoort area, near Pretoria in Gauteng, a number of horses died of suspected AHS. Virus isolation and typing was done from blood and/or organ samples of 21 suspected cases as well as from five zebra which were kept in the area. Virus was isolated from 14 of the 21 suspected cases but not from the zebra. The neutralizing antibody response of the zebra to the nine different African horsesickness virus (AHSV) serotypes was determined. Results indicated the highest prevalence of serotypes 2 and 4 followed by serotypes 1, 6 and 9. Reverse transcription polymerase chain reaction (RT-PCR) was performed on total RNA extracted from blood samples of the zebra. AHSV RNA was indicated in three of five zebra by agarose gel electrophoresis analysis of amplicons and in four of five zebra after Southern blot hybridization using a 32P-labelled probe. RT-PCR can be used together with serological techniques in studies of AHS to further clarify the epizootiology of the disease.

摘要

1995/1996年期间，南非部分地区降雨量异常高。人们观察到大量库蠓，随后爆发了非洲马瘟（AHS）。在豪登省比勒陀利亚附近的翁德斯特普特地区，一些马匹疑似死于非洲马瘟。对21例疑似病例的血液和/或器官样本以及该地区饲养的5匹斑马进行了病毒分离和分型。从21例疑似病例中的14例分离出了病毒，但斑马未分离出病毒。测定了斑马对9种不同非洲马瘟病毒（AHSV）血清型的中和抗体反应。结果表明，血清型2和4的流行率最高，其次是血清型1、6和9。对从斑马血液样本中提取的总RNA进行逆转录聚合酶链反应（RT-PCR）。通过对扩增产物的琼脂糖凝胶电泳分析，在5匹斑马中的3匹中检测到AHSV RNA，使用32P标记探针进行Southern印迹杂交后，在5匹斑马中的4匹中检测到AHSV RNA。在非洲马瘟研究中，RT-PCR可与血清学技术一起使用，以进一步阐明该病的流行病学。