Scherberich A, Beretz A
Pharmacologie et Physico-Chimie des Interactions Cellulaires et Moléculaires UMR CNRS 7034, Université Louis Pasteur Strasbourg I, Faculté de Pharmacie, Illkirch Graffenstaden, France.
Therapie. 2000 Jan-Feb;55(1):35-41.
Experimental models based on the culture of cells within tridimensional (3-D) gels add a 3-D organization to the classical 2-D culture of vascular cells. They allow the study of cell structure in an environment which is more representative of the in vivo situation and the investigation of cellular functions which cannot be studied using the basic 2-D models. This review shows examples of the use of cultures of vascular cells (endothelial cells, smooth muscle cells as well as fibroblasts) in 3-D collagen matrices for the study of cellular functions as diverse as angiogenesis, extracellular matrix reorganization, migration through 3-D collagen gels or phenotype modulation. It also describes recent advances in the in vitro reconstruction of biological blood vessels by bioengineering. A method for the preparation of 3-D collagen gels is described.
基于三维（3-D）凝胶中细胞培养的实验模型，为经典的血管细胞二维（2-D）培养增添了三维组织结构。它们使得在更能代表体内情况的环境中研究细胞结构成为可能，也能对使用基本二维模型无法研究的细胞功能展开研究。本综述展示了在三维胶原蛋白基质中培养血管细胞（内皮细胞、平滑肌细胞以及成纤维细胞）用于研究多种细胞功能的实例，这些功能包括血管生成、细胞外基质重组、通过三维胶原蛋白凝胶的迁移或表型调节。它还描述了通过生物工程在体外重建生物血管方面的最新进展。文中介绍了一种制备三维胶原蛋白凝胶的方法。
