You H, Wang B, Wang T
Beijing Friendship Hospital, Capital University of Medical Sciences, Beijing 100050, China.
Zhonghua Gan Zang Bing Za Zhi. 2000 Apr;8(2):78-80.
To investigate the proliferation and apoptosis of the hepatic stellate cell (HSC) in vitro and in vivo and the effects of Chinese herb, compound 861.
The in vitro study was carried out on the culture of hepatic stellate cell line. Various concentrations of compound 861 were added and incubated. Cell proliferation was detected with MTT colorimetric assay. Cell apoptosis was detected by electron microscopy, flow cytometry and TUNEL. The subjects of in vivo study were patients with chronic hepatitis B.
Compound 861 could significantly inhibit HSC proliferation and increase the apoptosis rate of HSC dose-dependently and time-dependently compared with the control group. After compound 861 incubation for 48h, the apoptosis rate of HSC was 25.9% compared with 9.2% in control group (P<0.05). The clinical study elucidated that the HSC was activated and proliferated in patients with chronic hepatitis B. After compound 861 treatment for 6 months, the number of activated HSC decreased and the apoptosis of HSC could be seen in the liver biopsy.
Apoptosis of activated HSC exists in vitro and in vivo, stimulating its apoptosis may play an important role in the treatment of liver fibrosis.
研究肝星状细胞（HSC）在体内外的增殖和凋亡情况以及中药复方861的作用。
体外实验采用肝星状细胞系培养，加入不同浓度的复方861进行孵育，用MTT比色法检测细胞增殖，通过电子显微镜、流式细胞术和TUNEL法检测细胞凋亡。体内实验对象为慢性乙型肝炎患者。
与对照组相比，复方861能显著抑制HSC增殖，并呈剂量和时间依赖性地增加HSC凋亡率。复方861孵育48小时后，HSC凋亡率为25.9%，而对照组为9.2%（P<0.05）。临床研究表明，慢性乙型肝炎患者的HSC被激活并增殖。复方861治疗6个月后，活化HSC数量减少，肝活检可见HSC凋亡。
活化HSC在体内外均存在凋亡现象，促进其凋亡可能在肝纤维化治疗中起重要作用。
