用单克隆抗体对远缘链球菌主要细胞表面蛋白抗原进行免疫标记。
Immunolabeling of the major cell surface protein antigen of Streptococcus sobrinus with monoclonal antibody.
作者信息
Fan M, Zhang P, Bian Z, Du M, Jin N
机构信息
College and Hospital of Stomatology, Hubei Medical University, Wuhan, P. R. China.
出版信息
Chin J Dent Res. 1999 May;2(2):23-6.
OBJECTIVE
The purpose of this study was to determine the accessibility of monoclonal antibody (McAb), specific for the major cell surface protein antigen (PAg) of Streptococcus sobrinus, to the surface of its native epitopes.
MATERIALS AND METHODS
An indirect immunogold labeling technique was used to detect the reaction of McAb with S. sobrinus 6715. The reactions of polyclonal antibodies (PcAbs) against S. sobrinus 6715 or PAg with S. sobrinus 6715, S. mutans Ingbritt C and S. rattus BHT were studied as controls.
RESULTS
The results indicated that PAg was localized on the outer cell surface of S. sobrinus, and McAb was reactive with only a few epitopes of the cell surface, whereas PcAbs were found to be reactive with more epitopes.
CONCLUSIONS
McAb was specific for the PAg, but there was cross-reaction with S. mutans. Also there seemed to be an association between the fuzzy coat on the surface of S. sobrinus and PAg.
目的
本研究旨在确定针对远缘链球菌主要细胞表面蛋白抗原（PAg）的单克隆抗体（McAb）与其天然表位表面的可及性。
材料与方法
采用间接免疫金标记技术检测McAb与远缘链球菌6715的反应。作为对照，研究了抗远缘链球菌6715或PAg的多克隆抗体（PcAbs）与远缘链球菌6715、变形链球菌Ingbritt C和鼠链球菌BHT的反应。
结果
结果表明，PAg定位于远缘链球菌的细胞外表面，McAb仅与细胞表面的少数表位反应，而PcAbs与更多表位反应。
结论
McAb对PAg具有特异性，但与变形链球菌存在交叉反应。此外，远缘链球菌表面的模糊被膜似乎与PAg有关。
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