López-López A, Castellote-Bargalló A I, López-Sabater M C
Departament de Nutrició i Bromatologia, Centre de Referència en Tecnologia dels Aliments, Facultat de Farmàcia, Universitat de Barcelona, Avinguda Joan XXIII s/n, Barcelona, 08028, Spain.
Anal Biochem. 2000 Jul 1;282(2):250-5. doi: 10.1006/abio.2000.4616.
We have validated and compared two direct methods for the determination of fatty acids in feces by capillary gas chromatography. Method I consisted of esterification of fatty acids using acetyl chloride. Method II used boron trifluoride-methanol as esterification reagent. The two methods were assayed with and without previous freeze-drying of the fecal sample. We found that the two methods could be carried out without sample freeze-drying. Precision and recovery rates were determined and the results were satisfactory. Both methods gave similar results, but Method II has certain advantages over Method I, such as speed, safety, and better recovery rates.
我们已经验证并比较了两种通过毛细管气相色谱法测定粪便中脂肪酸的直接方法。方法I是使用乙酰氯对脂肪酸进行酯化。方法II使用三氟化硼 - 甲醇作为酯化试剂。两种方法在粪便样品有无预先冷冻干燥的情况下进行测定。我们发现两种方法都可以在不进行样品冷冻干燥的情况下进行。测定了精密度和回收率，结果令人满意。两种方法给出的结果相似，但方法II比方法I具有某些优势，如速度、安全性和更好的回收率。
