人角质形成细胞中β-促脂解素样肽的条件依赖性存在。

Condition-dependent presence of beta-lipotropin-like peptide in human keratinocytes.

作者信息

Wintzen M, Zanello S B, Holick M F, Wiegant V M, Burbach J P, Vermeer B J

机构信息

Department of Dermatology, Leiden University Medical Center, Albinusdreef 2, 2333 ZA, Leiden, The Netherlands.

出版信息

Peptides. 2000 May;21(5):691-7. doi: 10.1016/s0196-9781(00)00193-5.

DOI:10.1016/s0196-9781(00)00193-5

PMID:10876052

Abstract

This study aimed to characterize the beta-endorphin-immunoreactive material (betaE-IR) detectable in normal human keratinocytes (NHK). The effects of different culturing conditions and UV-irradiation on production of betaE-IR by NHK were assessed by radioimmunoassay and HPLC. All culture systems contained low levels of betaE-IR that was increased in conditioned media after UV-irradiation under certain conditions. NHK grown in nutrient-poor medium contained highest levels of betaE-IR that exhibited beta-lipotropin-like properties after HPLC analysis. The other culturing conditions displayed no authentic betaE-related peptides. Our results indicate that under certain culturing conditions NHK can produce POMC peptides like beta-lipotropin, which can be induced by UV-radiation.

摘要

本研究旨在表征在正常人角质形成细胞（NHK）中可检测到的β-内啡肽免疫反应性物质（βE-IR）。通过放射免疫分析和高效液相色谱法评估不同培养条件和紫外线照射对NHK产生βE-IR的影响。所有培养系统中βE-IR水平较低，在某些条件下紫外线照射后的条件培养基中其水平会升高。在营养贫乏培养基中生长的NHK含有最高水平的βE-IR，高效液相色谱分析后显示其具有β-促脂素样特性。其他培养条件未显示出真正的βE相关肽。我们的结果表明，在某些培养条件下，NHK可以产生如β-促脂素这样的阿片促黑激素原肽，其可由紫外线辐射诱导产生。