[从噬菌体抗体库中筛选抗-HBsAg Fab噬菌体抗体]
[Selecting anti-HBsAg fab phage antibodies from the phage antibody library].
作者信息
Wang Z, Liu Q, Wan Z, Zhang D
机构信息
Institute for Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010, China.
出版信息
Zhonghua Gan Zang Bing Za Zhi. 2000 Jun;8(3):167-8.
OBJECTIVE
Selecting Fab phage antibodies to HBsAg from the phage antibody library.
METHODS
The phages displaying antibody fragments were subjected to three rounds of panning with HBsAg coated in solid phase.
RESULTS
The eluted phages were enriched nearly 80-fold, and the percentage of recombinant clones increased from 17% to 100% after the third round of panning. The HBsAg specific monoclonal antibodies were determined by ELISA. These antibodies combined specifically with HBsAg, but not with BSA and HAV antigens.
CONCLUSION
Our work on constructing human immunoglobulin combinatorial library and selecting anti-HBsAg Fab phage antibodies would be beneficial to the further study on the production of soluble Fab antibodies.
目的
从噬菌体抗体库中筛选抗乙肝表面抗原（HBsAg）的Fab噬菌体抗体。
方法
将展示抗体片段的噬菌体用固相包被的HBsAg进行三轮淘选。
结果
洗脱的噬菌体富集了近80倍，三轮淘选后重组克隆的比例从17%增加到100%。通过酶联免疫吸附测定（ELISA）确定HBsAg特异性单克隆抗体。这些抗体与HBsAg特异性结合，但不与牛血清白蛋白（BSA）和甲型肝炎病毒（HAV）抗原结合。
结论
我们构建人免疫球蛋白组合文库并筛选抗HBsAg Fab噬菌体抗体的工作将有助于进一步研究可溶性Fab抗体的生产。
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