白细胞介素-6启动子受与雌激素受体激素结合域融合的24 kDa成纤维细胞生长因子-2亚型调控。

IL-6 promoter is modulated by the 24 kDa FGF-2 isoform fused to the hormone binding domain of the oestrogen receptor.

作者信息

Delrieu I, Chinestra P, Delassus F, Bayard F, Prats H, Faye J C

机构信息

INSERM U397, Institut C. Regaud, Toulouse, France.

出版信息

Cytokine. 2000 Jul;12(7):1110-4. doi: 10.1006/cyto.1999.0693.

DOI:10.1006/cyto.1999.0693

PMID:10880259

Abstract

Fusion proteins consisting of the 24 kDa nuclear form of basic fibroblast growth factor (FGF-2), associated with the hormone binding domain of oestrogen receptor (HBD), convey oestrogen inducibility to FGF-2. When stable HBD-FGF-2 HeLa cell lines were transiently transfected with an interleukin 6 (IL-6) construct, the IL-6 promoter activity was downregulated by the addition of oestradiol. Moreover, in these cell lines, the function of the FGF-2 nuclear localisation sequence was abolished by its fusion to HBD, while addition of oestradiol restored the location of the chimera to the nucleus.

摘要

由24 kDa碱性成纤维细胞生长因子（FGF-2）的核形式与雌激素受体的激素结合域（HBD）组成的融合蛋白，可将雌激素诱导性传递给FGF-2。当稳定的HBD-FGF-2 HeLa细胞系用白细胞介素6（IL-6）构建体进行瞬时转染时，雌二醇的添加会下调IL-6启动子活性。此外，在这些细胞系中，FGF-2核定位序列与HBD融合后其功能被消除，而添加雌二醇可使嵌合体重新定位于细胞核。