Pino L A, Matinella L, Morales C G
Laboratorio de Parasitología, Instituto de Investigaciones Veterinarias, Centro Nacional de Investigaciones Agropecuarias, Venezuela.
Rev Soc Bras Med Trop. 1999 Nov-Dec;32(6):677-82. doi: 10.1590/s0037-86821999000600010.
Nine lots of 32 B. glabrata snails (5-7mm in diameter) from the following strains from the endemic area: Cagua, Valencia Lake (Puerta Negra Sector), Ingenio Bolívar (Aragua State), Mariara, Caserío El 25 and Güigüe (Carabobo state), and from the non-endemic area: Anzoátegui (Lara state), Chabasquén (Portuguesa state) and Caripe (Monagas state) were experimentally infected (5 miracidiums/snail) with C5 Schistosoma mansoni strain. The intramolluscal prepatent period oscillated between 23 and 25 days for the nine strains. The duration of intramolluscal infection varied a lot, from 20 days in the case of the Chabasquén strain to 93 days for the Güigüe strain. The average cercariae production in the 3rd day of emission varied from X = 74.4 for the Mariara strain to 591.7 in the case of the Chabasquén strain. Significant statistical differences were found in relation to the total number of emitted cercariae in the 3rd day (H = 97.4; P < 0.05), with significant statistical differences detected among most of the 36 strain combinations, except for the following: Mariara/Ingenio Bolívar, Cagua/Cas. El 25, Valencia Lake/Güigüe and Güigüe/Caripe. The strains of Valencia Lake (88.8%), Cagua (85.2), Chabasquén (82.6%) and Caripe (82.6%) showed the highest rates of self-cure, whereas the Güigüe strain showed the lowest: 21.4%.
从流行地区的以下品系采集了9批直径为5 - 7毫米的光滑双脐螺:卡瓜、巴伦西亚湖(黑门区)、玻利瓦尔糖厂(阿拉瓜州)、马里亚拉、卡西里奥埃尔25和圭圭(卡拉沃沃州),以及非流行地区的:安索阿特吉(拉腊州)、查瓦斯肯(葡萄牙萨州)和卡里佩(莫纳加斯州),用曼氏血吸虫C5株对其进行实验感染(每只螺5个毛蚴)。这9个品系的螺体内发育前期在23至25天之间波动。螺体内感染持续时间差异很大,查瓦斯肯品系为20天,而圭圭品系为93天。排尾第3天平均尾蚴产量从马里亚拉品系的X = 74.4到查瓦斯肯品系的591.7不等。在排尾第3天排出的尾蚴总数方面发现了显著的统计学差异(H = 97.4;P < 0.05),在36种品系组合中的大多数之间检测到显著的统计学差异,但以下组合除外:马里亚拉/玻利瓦尔糖厂、卡瓜/卡西里奥埃尔25、巴伦西亚湖/圭圭和圭圭/卡里佩。巴伦西亚湖品系(88.8%)、卡瓜品系(85.2%)、查瓦斯肯品系(82.6%)和卡里佩品系(82.6%)的自愈率最高,而圭圭品系最低,为21.4%。