采用高效液相色谱法测定虹鳟鱼（Oncorhynchus mykiss）和斑点叉尾鮰（Ictalurus punctatus）鱼片组织中的氯硝柳胺残留量。

Determination of niclosamide residues in rainbow trout (Oncorhynchus mykiss) and channel catfish (Ictalurus punctatus) fillet tissue by high-performance liquid chromatography.

作者信息

Schreier T M, Dawson V K, Choi Y, Spanjers N J, Boogaard M A

机构信息

Upper Midwest Environmental Sciences Center, U.S. Geological Survey, La Crosse, WI 54603, USA.

出版信息

J Agric Food Chem. 2000 Jun;48(6):2212-5. doi: 10.1021/jf990695r.

DOI:10.1021/jf990695r

PMID:10888524

Abstract

Bayluscide [the ethanolamine salt of niclosamide (NIC)] is a registered piscicide used in combination with 3-(trifluoromethyl)-4-nitrophenol (TFM) to control sea lamprey populations in streams tributary to the Great Lakes. A high-performance liquid chromatography (HPLC) method was developed for the determination of NIC residues in muscle fillet tissues of fish exposed to NIC and TFM during sea lamprey control treatments. NIC was extracted from fortified channel catfish and rainbow trout fillet tissue with a series of acetone extractions and cleaned up on C(18) solid-phase extraction cartridges. NIC concentrations were determined by HPLC with detection at 360 and 335 nm for rainbow trout and catfish, respectively. Recovery of NIC from rainbow trout (n = 7) fortified at 0.04 microg/g was 77 +/- 6.5% and from channel catfish (n = 7) fortified at 0.02 microg/g was 113 +/- 11%. NIC detection limit was 0.0107 microg/g for rainbow trout and 0.0063 microg/g for catfish. Percent recovery of incurred radioactive residues by this method from catfish exposed to [(14)C]NIC was 89.3 +/- 4.1%. Percent recoveries of NIC from fortified storage stability tissue samples for rainbow trout (n = 3) analyzed at 5 and 7.5 month periods were 78 +/- 5.1 and 68 +/- 2.4%, respectively. Percent recoveries of NIC from fortified storage stability tissue samples for channel catfish (n = 3) analyzed at 5 and 7.5 month periods were 88 +/- 13 and 76 +/- 21%, respectively.

摘要

贝螺杀（氯硝柳胺乙醇胺盐）是一种已注册的杀鱼剂，与3-（三氟甲基）-4-硝基苯酚（TFM）联合使用，用于控制五大湖支流溪流中的海七鳃鳗种群数量。开发了一种高效液相色谱（HPLC）方法，用于测定在海七鳃鳗控制处理期间接触贝螺杀和TFM的鱼类肌肉组织中的贝螺杀残留量。用一系列丙酮萃取法从强化的沟鲶和虹鳟鱼片组织中提取贝螺杀，并在C(18)固相萃取柱上进行净化。分别通过在360 nm和335 nm处检测的HPLC测定虹鳟和鲶鱼中的贝螺杀浓度。从添加量为0.04 μg/g的虹鳟（n = 7）中回收贝螺杀的回收率为77±6.5%，从添加量为0.02 μg/g的沟鲶（n = 7）中回收贝螺杀的回收率为113±11%。虹鳟的贝螺杀检测限为0.0107 μg/g，鲶鱼的检测限为0.0063 μg/g。用该方法从接触[(14)C]贝螺杀的鲶鱼中回收的放射性残留量的回收率为89.3±4.1%。在5个月和7.5个月期间分析的添加了贝螺杀的虹鳟（n = 3）储存稳定性组织样品中，贝螺杀的回收率分别为78±5.1%和68±2.4%。在5个月和7.5个月期间分析的添加了贝螺杀的沟鲶（n = 3）储存稳定性组织样品中，贝螺杀的回收率分别为88±13%和76±21%。