[人肺癌中nm23基因表达的Northern印迹分析]
[Northern blot analysis of nm23 gene expression in human lung cancer].
作者信息
Liu L, Qin Y, Zhou Q
机构信息
Department of Thoracocardiac Surgery, First University Hospital, West China University of Medical Sciences, Chengdu.
出版信息
Zhonghua Zhong Liu Za Zhi. 1998 Sep;20(5):342-4.
OBJECTIVE
To investigate the role of nm23 gene expression in lung cancer.
METHODS
Forty human lung cancer tissues and 19 non-cancerous pulmonary tissues were studied for their nm23-H1 and nm23-H2 mRNA expression with nonradioactive Northern blot hybridization. Correlation of nm23 mRNA expression with clinical features of lung cancer was analyzed.
RESULTS
The mRNA expression of nm23-H2 gene in poorly differentiated squamous-cell carcinoma was significantly decreased compared to that in moderately differentiated squamous-cell carcinoma. The mRNA expression of nm23-H1 and nm23-H2 genes in small cell lung cancer was decreased compared to that in squamous-cell carcinoma. No significant difference in nm23 mRNA expression was observed in lung cancers with and without lymph node metastasis, nor was there significant difference in patients with lung cancers in different stages.
CONCLUSION
The mRNA expression of nm23 gene is correlated with the degree of differentiation of lung cancer, but there is no evidence of metastasis suppression effect by nm23 gene.
目的
探讨nm23基因表达在肺癌中的作用。
方法
采用非放射性Northern印迹杂交法，研究40例人肺癌组织和19例非癌性肺组织中nm23-H1和nm23-H2 mRNA的表达情况。分析nm23 mRNA表达与肺癌临床特征的相关性。
结果
低分化鳞状细胞癌中nm23-H2基因的mRNA表达明显低于中分化鳞状细胞癌。小细胞肺癌中nm23-H1和nm23-H2基因的mRNA表达低于鳞状细胞癌。有无淋巴结转移的肺癌患者nm23 mRNA表达无显著差异，不同分期的肺癌患者也无显著差异。
结论
nm23基因的mRNA表达与肺癌的分化程度相关，但没有证据表明nm23基因具有转移抑制作用。
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