白细胞介素-18调节HIV-1 Nef DNA初免/蛋白加强疫苗诱导的免疫反应。

Interleukin-18 modulates immune responses induced by HIV-1 Nef DNA prime/protein boost vaccine.

作者信息

Billaut-Mulot O, Idziorek T, Ban E, Kremer L, Dupré L, Loyens M, Riveau G, Locht C, Capron A, Bahr G M

机构信息

Unité Inserm 167, Pasteur de Lille, Lille, France.

出版信息

Vaccine. 2000 Aug 15;19(1):95-102. doi: 10.1016/s0264-410x(00)00157-2.

DOI:10.1016/s0264-410x(00)00157-2

PMID:10924791

Abstract

Many different HIV-1 vaccine strategies have been developed, but as yet none has been completely successful. Promising results from combined DNA prime/protein boost vaccines have been reported. Specific immune responses generated by DNA vaccines can be modulated by the co-delivery of genes coding for cytokines. In this study, we have used the intradermal route by needle injection of a plasmid coding for the HIV-1 Nef accessory protein. We show that DNA prime/protein boost vaccine combinations increase the humoral and cellular immune responses against HIV-1 Nef and that the co-injection of DNA encoding Interleukin-18 (IL-18) modulates the specific immune response towards a Th1 type.

摘要

已经开发出许多不同的HIV-1疫苗策略，但迄今为止尚无一种完全成功。有报道称联合DNA初免/蛋白加强疫苗取得了有希望的结果。DNA疫苗产生的特异性免疫反应可通过共同递送编码细胞因子的基因来调节。在本研究中，我们采用皮内注射编码HIV-1 Nef辅助蛋白的质粒的方法。我们发现，DNA初免/蛋白加强疫苗组合可增强针对HIV-1 Nef的体液免疫和细胞免疫反应，并且共同注射编码白细胞介素-18（IL-18）的DNA可将特异性免疫反应调节为Th1型。